Class-Specific Protein Quantitation
Measure protein glycosylation as the percent of purified protein mass compared to included glycoprotein standards; uses periodic acid Schiff-base reagents (PAS).
- Qualitative—easily identifies purified proteins as glycoproteins or samples as contaminated with sugars
- Semiquantitative—estimates the percent carbohydrate content (w/w) of purified glycoprotein by comparison to the included set of glycoprotein standards
- Simple procedure—completed in less than 75 minutes; All you need is this kit, a microplate, and a plate reader to determine carbohydrate content
- Adaptable—standard curve format allows for the design of alternative tests for aldehydes and carbohydrate components
- Determine if a purified polyclonal antibody or other protein is glycosylated before attempting to perform carbohydrate-based conjugation or immobilization chemistries with hydrazide reagents
- Characterize and classify purified protein fractions from an affinity procedure
- Assess sugar and carbohydrate contamination in non-glycosylated protein samples
Classify and identify proteins as phosphorylated (serine and threonine) and obtain semi-quantitative assessment of the phosphorylation levels.
- Specific—measures phosphoserine (p-Ser) and phosphothreonine (p-Thr) only; does not measure phosphotyrosine (p-Tyr)
- Convenient—test tube and 96-well microplate protocols included and requires less than 90 minutes to perform
- Semi-quantitative—calculate moles of phosphate (P) per mole of protein using the included phosvitin standard
- Customizable—format is adaptable to development of specific assays for pure phosphoproteins when previously characterized standards are available
- Identify proteins as containing phosphoserine or phosphothreonine phosphorylations
- Estimate the amount of pS and pT phosphorylation
- Develop specific quantitative assays for well-characterized phosphoproteins of interest
For Research Use Only. Not for use in diagnostic procedures.