These specialized protein assay reagents and kits allow you to estimate and measure specific post-translational modification (PTM) or functional (enzymatic) classes of proteins in biological samples, including glycoproteins and phosphoproteins.
23260-Glyco-Carbo-Kit-250
Glycoprotein Carbohydrate Estimation Kit

Measure protein glycosylation as the percent of purified protein mass compared to included glycoprotein standards; uses periodic acid Schiff-base reagents (PAS). 

Assay overview

  • Qualitative—easily identifies purified proteins as glycoproteins or samples as contaminated with sugars
  • Semiquantitative—estimates the percent carbohydrate content (w/w) of purified glycoprotein by comparison to the included set of glycoprotein standards
  • Simple procedure—completed in less than 75 minutes; All you need is this kit, a microplate, and a plate reader to determine carbohydrate content
  • Adaptable—standard curve format allows for the design of alternative tests for aldehydes and carbohydrate components

Applications

  • Determine if a purified polyclonal antibody or other protein is glycosylated before attempting to perform carbohydrate-based conjugation or immobilization chemistries with hydrazide reagents
  • Characterize and classify purified protein fractions from an affinity procedure
  • Assess sugar and carbohydrate contamination in non-glycosylated protein samples
Phosphoprotein Phosphate Estimation Assay Kit

Classify and identify proteins as phosphorylated (serine and threonine) and obtain semi-quantitative assessment of the phosphorylation levels.

Assay overview

  • Specific—measures phosphoserine (p-Ser) and phosphothreonine (p-Thr) only; does not measure phosphotyrosine (p-Tyr)
  • Convenient—test tube and 96-well microplate protocols included and requires less than 90 minutes to perform
  • Semi-quantitative—calculate moles of phosphate (P) per mole of protein using the included phosvitin standard
  • Customizable—format is adaptable to development of specific assays for pure phosphoproteins when previously characterized standards are available

Applications

  • Identify proteins as containing phosphoserine or phosphothreonine phosphorylations
  • Estimate the amount of pS and pT phosphorylation
  • Develop specific quantitative assays for well-characterized phosphoproteins of interest
23270-Phospho-Estimate-250

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