82036-qIP-Magnetic-Myc-Kit-250

Our qIP Protein Interaction Kits use anti-HA or anti-Myc beads (agarose or magnetic) and a sensitive luciferase assay system to co-immunoprecipitate (co-IP) and quantify interactions between epitope-tagged and TurboLuc luciferase enzyme (Tluc) tagged protein pairs expressed in mammalian cells. The quantitative immunoprecipitation (qIP) system uses Tluc enzyme to accurately and precisely reflect the abundance of a specific co-IP product, to help avoid the need for time-consuming gel electrophoresis, western blotting, and band densitometry.

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Featured qIP products

An agarose bead format of our qIP kit, using HA tagging

Exploit the convenience of magnetic bead-mediated qIP

Recover and quantify proteins that bind to your Myc-tagged bait protein

Magnetic beads make quantitative immunoprecipitation fast and robust

Features of qIP kits

  • Quantitative—integrated luciferase assay enables direct measurement of co-IP products, and control system helps ensure accuracy and normalization
  • Sensitive—bright bioluminescence signal allows detection of weak interactions
  • Time-saving—final read-out is relative luminescence units (RLU), rather than  elution and western blotting
  • Simple and fast—incubation and wash steps use spin columns or microcentrifuge tubes
  • Robust—vectors, kit, and qIP method is compatible with various  mammalian cell lines (293T, HEK293, NIH3T3, and CHO-K1)
  • Versatile—highly customizable assay platform :
    • Additional cloning vectors are available for N- and C-terminal fusions
    • HA- and c-Myc tag kits are available in both agarose and magnetic bead formats
    • Assay reagents and buffers are available separately 

Schematic of the qIP assay procedure

qIP-002-Generic-Protocol