We offer Luciferase reporter reagents, multiple cloning site vectors, and flash, glow and dual-spectral luciferase assay kits based on the intracellularly expressed firefly luciferase. Firefly luciferase is a 60kDa protein produced in nature by several species of the Lampyridae family of beetles which includes the genera Photinus and Luciola. Bioluminescent signal from firefly luciferase originates from the oxidation of D-luciferin. Light output captured using a luminometer can be correlated with the amount of firefly luciferase protein produced and used to determine the activity of the promoter driving firefly expression.

Choose the right Firefly luciferase assay for your application

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Renilla Luciferase Flash Assay Kits

Pierce™ Firefly Luciferase Flash Assay Kits

Renilla Luciferase Glow Assay Kits

Pierce™ Firefly Luciferase Glow Assay Kits

Renilla Luciferase Dual Assay Kits

Pierce™ Firefly Luc One-Step Glow Assay Kits
Spectral Type Single-spectral Single-spectral Single-spectral
Assay Type Flash-type Glow-type Flash-type
Luciferase Firefly Firefly Firefly
Description
  • Flash-type assays that provide an intense but short burst of light for maximum sensitivity
  • May require the use of a luminometer with reagent injectors for the greatest signal strength and reduced variability
  • Glow-type assays that provide a less intense but longer-duration signal that is amenable to higher sample throughput
  • Do not require reagent injectors
  • Automation friendly – amenable to high throughput assays
  • One-step, mix-and-read protocol; no additional reagents required
  • Glow-type assays that provide a less intense but longer-duration signal that is amenable to higher sample throughput
  • Do not require reagent injectors
Average Half-Life 30 min >1 hr >1 hr

The Thermo Scientific™ Pierce™ Firefly Luciferase Flash Assay Kit provides reagents to measure Red firefly luciferase activity in whole cell producing a very strong flash signal which decays rapidly.

The Thermo Scientific™ Pierce™ Firefly Luciferase Glow Assay Kit provides a bright and stable bioluminescence signal (half-life greater than 2 hours) in the presence of firefly luciferase reporters for protein expression assays.


Red firefly luciferase reporter assay vectors


Firefly luciferase reaction

Firefly luciferase reaction