22860-PVDF-Membrane-F-230

Polyvinylidene difluoride (PVDF) membrane is ideal for western blotting applications as well as for amino acid analysis and protein sequencing of small amounts of proteins (as little as 10 pmoles). In addition, PVDF membranes can be used stripped and reprobed without a loss of sensitivity or increased background.

PVDF membranes are highly hydrophobic and must be pre-wetted with methanol or ethanol prior to submersion in transfer buffer. PVDF membranes have a high binding affinity for proteins and nucleic acids and may be used for applications such as western, Southern, northern, and dot blots; binding likely occurs via dipole and hydrophobic interactions. PVDF membranes offer a better retention of adsorbed proteins than other supports because of the greater hydrophobicity. PVDF is also less brittle than nitrocellulose.

While our 0.2 μm PVDF membrane performs well for Western blotting, amino acid analysis, and protein sequencing  applications, our high-quality Invitrolon™ PVDF membrane (0.45 µm) is particularly suited for high sensitivity and low background immunoblotting. The Invitrolon™/ Filter Paper Sandwich includes a high-quality PVDF membrane ideal for blotting proteins from E-PAGE™ Gels or similar dimension gels. The major features of the Invitrolon™/Filter PVDF Membrane Paper Sandwich include:

  • High sensitivity and low background blotting
  • Contains no support fabric or detergents
  • Compatible with commonly used transfer conditions and detection methods such as staining, immunodetection, fluorescence, or radiolabeling
  • Supplied in a precut, pre-assembled membrane/filter paper sandwich to fit E-PAGE™ gels and other comparable gel sizes

If you are using our iBlot® Dry Blotting System for your western blot transfer, you will want to use our iBlot® PVDF Transfer Stacks.

Choose our low-fluorescence membrane (0.2 µm) for fluorescent blotting.

Applications and specifications for PVDF membranes

Specs Invitrolon™ PVDF 0.2 µm PVDF Precut PVDF membrane PVDF rolls
Cat # LC2005
(20 transfers)
LC2002
(20 transfers)
88585
(10 transfers)
22860
(10 transfers)
88518
(111 transfers)
88520
(111 transfers)
Quant. 20 membrane / filter paper sandwiches 20 membrane / filter paper sandwiches 10 Pre-cut PVDF sheets 10 Pre-cut PVDF sheets 1 roll
111 sheets (8 x 10cm)
1 roll
111 sheets (8 x 10cm)
Pore size 0.45 µm 0.2 µm 0.45 µm 0.2 µm 0.45 µm 0.2 µm
Dimensions 8.3cm x 7.3cm 8.3cm x 7.3cm 10cm x 10cm 7cm x 8.4cm 26.5cm x 3.75m roll 26.5cm x 3.75m roll
Binding capacity Goat IgG: 294 µg/cm2,
BSA: 131 µg/cm2,
Insulin: 85 µg/cm2
50–150 µg/cm2 for large, globular proteins,
>150 µg/cm2 for smaller peptides
Goat IgG: 294 µg/cm2,
BSA: 215 µg/cm2,
Insulin: 160 µg/cm2
  Goat IgG: 294 µg/cm2,
BSA: 215 µg/cm2,
Insulin: 160 µg/cm2
Goat IgG: 448 µg/cm2,
BSA: 340 µg/cm2,
Insulin: 262 µg/cm2
Application - Optimal western blot transfers for proteins >10 kDa
- Protein sequencing
- Amino acid analysis
- Other western blot transfers
- Protein sequencing
- Amino acid analysis
- Solid - phase assay systems
- Western blot transfers
- Protein sequencing
- Amino acid analysis

- Fluorescence western blotting

- Western blot transfers
- Protein sequencing
- Amino acid analysis
- Western blot transfers
- Protein sequencing
- Amino acid analysis
Reprobe characteristics Yes Yes Yes Yes Yes Yes