The Thermo Scientific™ Pierce™ Protein Methods library is an encyclopedia of articles about protein analysis laboratory techniques.

The continually expanded and updated library is a valued resource for new and experienced researchers alike. Are you new to the protein biology laboratory and need a basic introduction to protein analysis techniques? Are you a seasoned scientist considering how to optimize an experiment involving protein expression, purification or detection? Pierce Protein Methods can help you. In addition, this educational content will assist you in choosing appropriate products from our portfolio to successfully accomplish your experiments.


Featured protein methods articles

Western Blot Video Tutorials

Western blotting is a multi-step process that can take 1–2 days to complete, depending on the methods used. These western blot video tutorials detail the common steps to perform a western blot, from separating proteins in a lysate to developing the blot.

Chemistry of Crosslinking

Crosslinking reagents (or crosslinkers) are molecules that contain two or more reactive ends capable or chemically attaching to specific functional groups (primary amines, sulfhydryls, etc.) on proteins or other molecules. This article describes the chemistry and variety of crosslinkers that exist.

Quantitative Proteomics

This article outlines various strategies for quantitative proteomic analyses, including those for relative quantitation (SILAC, ICAT, ICPL and isobaric tags) and for absolute quantitation (via SRM), and both labeled and label-free techniques.


Quick links to method overviews

The table at the bottom of this page contains links to both method articles and method overview articles. If a method overview is what you're looking for, links to those are provided separately below.


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Protein Method Article Title Protein Method Categories
An Overview of Cell Lysis and Protein Extraction Cell Lysis and Fractionation
Traditional Methods of Cell Lysis Cell Lysis and Fractionation
Detergents for Cell Lysis and Protein Extraction Cell Lysis and Fractionation
Cell Lysis Solutions Cell Lysis and Fractionation
Cell Fractionation and Organelle Isolation Cell Lysis and Fractionation
Protease and Phosphatase Inhibitors Cell Lysis and Fractionation
An Overview of Affinity Purification Protein Purification
Covalent Immobilization of Affinity Ligands Protein Purification
GST-tagged Proteins–Production and Purification Protein Purification
His-tagged Proteins–Production and Purification Protein Purification
Immunoprecipitation (IP) Protein Purification
An Overview of Buffer Exchange Dialysis, Desalting, and Concentration
Dialysis Methods for Protein Research Dialysis, Desalting, and Concentration
Desalting and Gel Filtration Chromatography Dialysis, Desalting, and Concentration
An Overview of Protein Assays Protein Assays
Chemistry of Protein Assays Protein Assays
Protein Assay Data Analysis Protein Assays
Protein-Protein Variation of Protein Assays Protein Assays
An Overview of Electrophoresis Protein Gel Electrophoresis
Protein Gel Stains Protein Gel Electrophoresis
An Overview of Detection Probes Detection Probes, ELISA, Western Blotting
Secondary Antibodies as Probes Detection Probes, ELISA, Western Blotting
Enzyme Probes Detection Probes, ELISA, Western Blotting
Avidin-Biotin Interaction Detection Probes, ELISA, Western Blotting, Protein Purification
Fluorescent Probes Detection Probes, Immunohistochemistry
Luciferase Reporters Reporter Gene Assays
An Overview of Western Blotting Western Blotting
Chemiluminescent Western Blotting Western Blotting
Stripping and Reprobing Western Blots Western Blotting
Fluorescent Western Blotting Western Blotting
Blocking Buffers for Western Blot and ELISA Western Blotting
Western Blot Transfer Methods Western Blotting
Chromogenic Western Blotting Substrates Western Blotting
Western Blot Video Tutorials Western Blotting
An Overview of ELISA ELISA
ELISA Development and Optimization ELISA
An Overview of Immunohistochemistry Immunohistochemistry
Immunohistochemistry vs Immunocytochemistry Immunohistochemistry
Fixation Strategies and Formulations Immunohistochemistry
Paraffin Removal and Antigen Retrieval Immunohistochemistry
Methods to Block Endogenous Detection Immunohistochemistry
Blocking Strategies for IHC Immunohistochemistry
IHC Immunodetection Immunohistochemistry
Avidin-Biotin Complex Method for IHC Detection Immunohistochemistry
Immunofluorescence Method for IHC Detection Immunohistochemistry
IHC Counterstains Immunohistochemistry
IHC Troubleshooting Guide Immunohistochemistry
An Overview of Protein-Protein Interaction Analysis Protein-Protein Interactions
Co-Immunoprecipitation (Co-IP) Protein-Protein Interactions
Pull-Down Assays Protein-Protein Interactions
Crosslinking Protein Interaction Analysis Protein-Protein Interactions
Label Transfer Protein Interaction Analysis Protein-Protein Interactions
Far-Western Blot Analysis Protein-Protein Interactions
An Overview of Protein-Nucleic Acid Interactions Protein-Nucleic Acid Interactions
Gel Shift Assays (EMSA) Protein-Nucleic Acid Interactions
Methods for Detecting Protein-DNA Interactions Protein-Nucleic Acid Interactions
Methods for Detecting Protein-RNA Interactions Protein-Nucleic Acid Interactions
Chromatin IP (ChIP Assays) Protein-Nucleic Acid Interactions
Methods for Labeling Nucleic Acids Protein-Nucleic Acid Interactions
An Overview of Mass Spectrometry Protein Mass Spectrometry Analysis
Quantitative Proteomics Protein Mass Spectrometry Analysis
Sample Preparation for Mass Spectrometry Protein Mass Spectrometry Analysis
An Overview of Protein Labeling Protein Labeling
Biotinylation Protein Labeling
Metabolic Labeling and Chemoselective Ligation Protein Labeling
An Overview of Crosslinking and Protein Modification Protein Crosslinking
Chemistry of Crosslinking Protein Crosslinking
Crosslinking Applications Protein Crosslinking
Polyethylene Glycol (PEG) and Pegylation of Proteins Protein Crosslinking
Carbodiimide Crosslinker Chemistry Protein Crosslinking
Amine-Reactive Crosslinker Chemistry Protein Crosslinking
Sulfhydryl-Reactive Crosslinker Chemistry Protein Crosslinking
Carbonyl-Reactive Crosslinker Chemistry Protein Crosslinking
Photoreactive Crosslinker Chemistry Protein Crosslinking
Staudinger Ligation Reaction Chemistry Protein Crosslinking
An Overview of Antibody Production and Purification Antibody Production and Purification
Antibody Production (Immunogen Preparation) Antibody Production and Purification
Antibody Isotyping and Characterization Methods Antibody Production and Purification
Antibody Purification Methods Antibody Production and Purification
Antibody IgG Binding Proteins Antibody Production and Purification
Antibody Fragmentation Antibody Production and Purification
Antibody Structure and Classes Antibody Production and Purification
Antibody Labeling and Immobilization Sites Antibody Production and Purification
An Overview of Post-Translational Modification Post-Translational Modification
Phosphorylation Post-Translational Modification
Glycosylation Post-Translational Modification
An Overview of Protein Expression Systems Protein Expression
Cell-Free Protein Expression Protein Expression
Peptide Design Peptides
Peptide Synthesis Peptides
Amino Acid Physical Properties Peptides