Introducing a Western Blotting Substrate that is Chemiluminescent and Chemifluorescent
Detect your target by film, CCD cameras or laser-based imagers
by Thermo Scientific Pierce Protein Biology Team - 06/01/11
The Thermo Scientific Pierce ECL Plus Substrate is a chemiluminescent and chemifluorescent peroxidase substrate for western blotting. The Pierce ECL Plus Substrate generates acridinium esters when it reacts with horseradish peroxidase (HRP), producing a strong signal that is equal to or better than similar commercially available substrates (Figure 1). The ester intermediates react with peroxide and produce a strong and sustained chemiluminescent signal that can be captured by X-ray film or a CCD camera. A robust fluorescent signal is also produced, which can be captured with fluorescence imagers (Figure 2).
Pierce ECL Plus Substrate enables detection of low-picogram amounts of target protein (Figure 3) on nitrocellulose and PVDF membranes when probed with the appropriate primary and secondary antibody concentrations. This versatile and reliable western blotting substrate has a broad dynamic range and excellent sensitivity combined with a long-lasting signal (Figure 4).
For Research Use Only. Not for use in diagnostic procedures.