New Dimensions for Low-volume Sample Dialysis
Self-contained devices give you the solution for high-protein recovery
by Thermo Scientific Pierce Protein Biology Team - 06/01/11
The Thermo Scientific Slide-A-Lyzer MINI Dialysis Devices are a convenient and easy means for dialyzing small-volume protein samples (10-100μL). Our new the 0.5mL and 2mL Slide-A-Lyzer* MINI Dialysis Devices can process 50-500μL and 200-2000μL samples, respectively, and are self-contained, single-use devices that require no syringes, centrifuge, beakers or laborious steps. Sample addition and removal are easily accomplished using standard laboratory pipettes. Dialysis is rapidly and efficiently performed directly in the supplied 15mL or 50mL conical tubes, minimizing waste and the amount of dialysis buffer required. Multiple samples can be easily processed simultaneously and safely. The capped devices can be placed in standard laboratory racks or shakers, saving space and minimizing the risk of spills or contamination.
Dialysis is a commonly used technique for separating sample components based on selective diffusion across a porous membrane. The pore size determines the MWCO of the membrane, which is defined as the molecular weight at which 90% of a typical protein or polypeptide is retained by the membrane. The Slide-A-Lyzer MINI Dialysis Devices are made of low-binding polypropylene and integrated regenerated cellulose membranes with MWCO specifications of 3.5K, 10K or 20K. Regenerated cellulose membranes are compatible with a wide variety of common chemicals and buffers and provide excellent protein recovery (Table 1) and dialysis rates (Figure 1). Slide-A-Lyzer MINI Dialysis Devices are manufactured in a HEPA filtered clean-room facility, and 100% of units are vacuum-leak tested to ensure the highest quality and safety for your samples.
Table 1. High protein recovery is obtained using the 2mL dialysis device.†
|Membrane MWCO (kDa)||Protein/Peptide||Recovery (%)|
|3.5||Insulin Chain B (3.5kDa)||90.13|
|10||Cytochrome C (12.4kDa)||94.44|
|† Insulin chain B, cytochrome C and myoglobin (0.25mg/mL) in either 50mM sodium phosphate, 75mM NaCl at pH 7.2 or 0.2M carbonate-bicarbonate buffer at pH 9.4 were dialyzed overnight (17 hours) at 4°C. The amount of protein in the retentate was determined using the Thermo Scientific Pierce BCA Protein Assay (Part No. 23225).|
Using the Slide-A-Lyzer MINI Dialysis Devices, low-molecular weight contaminant removal, buffer exchange and desalting can be accomplished within 4-8 hours using the procedure outlined in Figure 1 with only 30-100mL of buffer. Dialysis efficiencies, rates and recoveries are similar to conventional dialysis using a large volume of buffer (Figure 2).
For Research Use Only. Not for use in diagnostic procedures.