Cell-FreeProteinExpression

We have several optimized cell-free expression systems for the rapid synthesis of recombinant proteins utilizing rabbit reticulocyte, CHO-, or HeLa-based lysate systems. These mammalian systems can produce full-length and functionally active proteins, with the CHO and HeLa systems typically expressing higher protein yields than rabbit reticulocyte system.

  • More choices—a variety of mammalian expression systems to accommodate different vectors, tags, yield requirements, functionality needs and cost considerations
  • Fast—proteins can be expressed in as little as 90 minutes
  • Easy-to-use—kits include all reagents and accessories needed for protein expression experiments
  • High performance—kits are designed to maximize protein yield and/or function compared to similar products from other vendors

Featured mammalian cell-free protein expression products

1-StepHumanHighYieldIVTKits
Multiple-Affinity-Tags-Purify-Expressed-Protein
pT7CFE1-CGST-HA-His-Vector

Choose the right mammalian protein expression system for your experiment

  Retic Lysate IVT™ Kit 1-Step Human Coupled IVT Kit 1-Step Human High Yield IVT Kit 1-Step CHO High Yield IVT Kit
Source Rabbit reticulocyte HeLa lysate HeLa lysate CHO (Chinese hamster ovary) lysate
Yield Low Medium High High
Protein modifications Limited glycosylation Glycosylation and phosphorylation Glycosylation and phosphorylation Glycosylation and phosphorylation
Reaction time 90 min 90 min to 6 hr 6–24 hr 6–24 hr
Comments Flexible system First human-based system Higher protein yield per reaction Most economical cost per milligram produced
Catalog # 60 rxn: AM1200,
60 rxn w/manual AM1200M
DNA: 88881, 88882
mRNA: 88883, 88884
Mini: 88890, 88891
Maxi: 88892
88893, 88894

T7 cell-free expression vectors

Thermo Scientific™ T7 Cell-Free Expression Vectors (pT7CFE1) are cloning plasmids optimized for use with the Thermo Scientific 1-Step Human In Vitro Protein Expression System. They are used for the in vitro translation (IVT) of genes, into functional proteins when used in combination with the 1-Step Human IVT system. The pT7CFE1 Vector is available with single or tandem affinity tags at the N- or C- terminus to facilitate protein purification and detection.

Ordering information for T7 cell-free expression vectors