Mammalian Cell-Free Protein Expression
We have several optimized cell-free expression systems for the rapid synthesis of recombinant proteins utilizing rabbit reticulocyte, CHO-, or HeLa-based lysate systems. These mammalian systems can produce full-length and functionally active proteins, with the CHO and HeLa systems typically expressing higher protein yields than rabbit reticulocyte system.
- More choices—a variety of mammalian expression systems to accommodate different vectors, tags, yield requirements, functionality needs and cost considerations
- Fast—proteins can be expressed in as little as 90 minutes
- Easy-to-use—kits include all reagents and accessories needed for protein expression experiments
- High performance—kits are designed to maximize protein yield and/or function compared to similar products from other vendors
Featured mammalian cell-free protein expression products
Choose the right mammalian protein expression system for your experiment
|Retic Lysate IVT™ Kit||1-Step Human Coupled IVT Kit||1-Step Human High Yield IVT Kit||1-Step CHO High Yield IVT Kit|
|Source||Rabbit reticulocyte||HeLa lysate||HeLa lysate||CHO (Chinese hamster ovary) lysate|
|Protein modifications||Limited glycosylation||Glycosylation and phosphorylation||Glycosylation and phosphorylation||Glycosylation and phosphorylation|
|Reaction time||90 min||90 min to 6 hr||6–24 hr||6–24 hr|
|Comments||Flexible system||First human-based system||Higher protein yield per reaction||Most economical cost per milligram produced|
|Catalog #||60 rxn: AM1200,
60 rxn w/manual AM1200M
|DNA: 88881, 88882
mRNA: 88883, 88884
|Mini: 88890, 88891
T7 cell-free expression vectors
Thermo Scientific™ T7 Cell-Free Expression Vectors (pT7CFE1) are cloning plasmids optimized for use with the Thermo Scientific 1-Step Human In Vitro Protein Expression System. They are used for the in vitro translation (IVT) of genes, into functional proteins when used in combination with the 1-Step Human IVT system. The pT7CFE1 Vector is available with single or tandem affinity tags at the N- or C- terminus to facilitate protein purification and detection.
Ordering information for T7 cell-free expression vectors
For Research Use Only. Not for use in diagnostic procedures.