Membrane-pro-kits

For researchers studying G protein–coupled receptors (GPCRs) and other membrane proteins, MembranePro™ expression systems deliver enriched, functional membrane proteins efficiently and reliably.

  • Proteins are displayed on mammalian cell membranes
  • Cellular quality control and mammalian posttranslational processing help produce functional proteins
  • Proteins bud off on lipoparticles and are easily collected from the culture medium
  • Lipoparticles are enriched with expressed receptors
Choose the MembranePro™ expression system that's right for your experiment
Adherent culture Suspension culture
  MembranePro™ Functional Protein Expression Kit NEW Expi293™ MembranePro™ Expression System
Benefits

The MembranePro™ Functional Kit provides:

  • Easier prep (less labor/hands-on time)
  • Higher receptor density, more homogeneous final product
  • High-density replacement for cell membranes in receptor–ligand binding studies

In addition to the benefits of the MembranePro™ FPE Kit, the Expi293™ MembranePro™ Expression System provides:

  • >20-fold increase in membrane protein yield compared to the adherent culture system*
  • Ease of use—no enzymatic or mechanical dissociation required
  • Scalability—easy scale-up without the need for multiple flasks
Typical total protein
yield for ~30 mL reaction
50–500 µg/mL >1 mg/mL
Kit contents
  • MembranePro™ Reagent
  • MembranePro™ Precipitation Mix
  • Lipofectamine® 2000 Transfection Reagent
  • pEF6 V5-His TOPO® Expression Vector Kit
  • 293FT cells
  • MembranePro™ Reagent
  • MembranePro™ Precipitation Mix
  • ExpiFectamine™ 293 Transfection Kit
  • Expi293™ Expression Medium
  • Opti-MEM® I Reduced-Serum Medium
Other required components
(to purchase separately)
Protocol Download protocol Download protocol
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**Actual membrane protein yield will vary with protein expressed

Resources

Have any questions on nucleic acid purification in your experiment? Click below to access FAQs, tech notes, selections guides, citations, and scientific presentations.

NEW Protein Expression Support Center
Find tips, troubleshooting help, and resources for your Protein Expression applications.