Precise-Protein-Gels

Precise™ Tris-Glycine Gels are long shelf-life, fast run-time polyacrylamide gels for SDS-PAGE that use the popular Laemmli buffer system and commercial gel tanks to help provide outstanding protein resolution and separation.

Precise™ Tris-Glycine Gels are cast in two sizes: durable 10 x 10 cm plastic cassettes that fit in Novex® XCell gel tanks, 10 x 8 cm plastic cassettes that fit in Mini-PROTEAN™ gel tanks. The gels are individually packaged in easy-to-open plastic pouches and are ready for electrophoresis with no comb or tape removal required. Following electrophoresis, the easy-open cassette design minimizes gel damage and allows gels to be removed without special tools. Precise Gels in the 10 x 10 cm and 10 x 8 cm sizes are available in gradient (4 to 20% and 8 to 16%) and fixed (8%, 10% and 12%) acrylamide concentrations and in 10-well, 12-well and 15-well formats.

Precise Tris-Glycine gel features

  • High-performance–Tris-Glycine running buffer helps produce excellent separation and high-resolution protein bands
  • Long shelf-life–up to 12-month stability helps ensures consistent performance for the duration of each package of gels
  • Easy to load–sample wells reinforced with plastic to minimize damage when loading and well-to-well contamination

In addition to Precise Tris-Glycine gels, we offer many other minigels in various formulations. Find the right one for your experiment using our interactive gel selection tool.

Specifications of Precise Tris-Glycine gels

  • Cassette dimensions: 10 cm x 8.0 cm x 5 mm or
    10 cm x 10 cm x 7 mm
  • Gel dimensions: 8 cm x 6.8 cm x 1 mm or
    8 cm x 8.8 cm x 1 mm
  • Storage conditions: up to 1 year at 4°C
  • Stacking gel: 4% polyacrylamide
  • Buffer system in gel: Tris-HCl, pH 7
  • SDS content: none
  • Required running buffer: Tris-glycine-SDS
  • Recommended sample buffer: Tris-HCl-SDS or LDS-SDS

Compatible gel tanks

  • Bio-Rad™ Mini-PROTEAN™ II and 3
  • Bio-Rad Mini-PROTEAN Tetra Cell
  • Hoefer™ Tall Mighty Small™ (SE 280)
  • Hoefer™ Mighty Small II (SE 260)
  • IBI Scientific Universal Protein Systems
  • Thermo Fisher Scientific Novex™ XCell I, XCell II and XCell II SureLock™

Precise Tris-Glycine gel selection table

Figure 1: Protein migration table of the various gels.

Video: Separating proteins by SDS-PAGE