_26139-EZ-Link-Alkoxy-PEG12-230

The carbohydrate portion of glycoproteins is one of the most common targets for protein modification. It can be reacted with hydrazide derivatives of biotin. This approach is useful for antibodies because they become biotinylated in a manner that maintains immunological reactivity; particularly polyclonal antibodies because they are heavily glycosylated. Monoclonal antibodies may be deficient in glycosylation and success with this method will depend on the extent of glycosylation for a particular antibody.

Choose the best carbonyl biotinylation reagent for your experiment

Reagent category Order products Product features (description) Spacer arm (Å) Water  soluble? Cleavable? Membrane permeable?
Alkoxyamine-PEG12-Biotin Glycoprotein biotinylation via a very long polyethylene glycol (PEG) spacer arm
55.4 Yes  No  No
Alkoxyamine-PEG4-Biotin Glycoprotein biotinylation via a long polyethylene glycol (PEG) spacer arm 31.3  Yes No  No 
Alkoxyamine-PEG4-SS-PEG4-Biotin Glycoprotein biotinylation via a cleavable pegylated spacer arm  56.6 Yes Yes No
Hydrazide-Biocytin Can be used to label DNA and RNA through cytosine residues 19.7  No No  No 
Hydrazide-Biotin Reagents Short and mid-length reagents for labeling glycoproteins and other carbohydrate-containing compounds having oxidizable sugars or aldehydes
15.7 No No Yes 
Hydrazide-Biotin Reagents Short and mid-length reagents for labeling glycoproteins and other carbohydrate-containing compounds having oxidizable sugars or aldehydes 24.7 No No Yes
Hydrazide-PEG4-Biotin A carbohydrate-biotinylation reagent with a long, hydrophilic spacer arm 31.3 Yes No No
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