Sulfhydryl-reactive biotinylation reagents

Sulfhydryl groups are the second most common target for modification in biological studies. They are found as exposed cysteines in protein and peptide. Sulfhydryl group biotinylation provides an advantage in some applications; for example, targeting a sulfhydryl group can help preserve the biological activity of an enzyme when amines are found at the active site.

Choose the right sulfhydryl biotinylation reagent for your experiment

Product categories Product Product features Spacer arm (Å) Water  soluble? Cleavable? Membrane permeable?
BMCC-Biotin EZ-Link BMCC-Biotin A cysteine-biotinylation reagent with an extended cyclohexane spacer arm 32.6 No No Yes
HPDP-Biotin EZ-Link HPDP-Biotin  For reversible biotinylation of cysteine sulfhydryl groups 29.2 No Yes Yes
Iodoacetyl-LC-Biotin

EZ-Link Iodoacetyl-LC-Biotin 

Haloacetyl-activated biotinylation reagent for sulfhydryl labeling 27.1 No No Yes
Iodoacetyl-PEG2-Bioti

EZ-Link Iodoacetyl-PEG2-Biotin

Haloacetyl-activated, water-soluble biotinylation reagent for sulfhydryls 24.7 Yes No No
Maleimide-PEG11-Biotin

EZ-Link Maleimide PEG11-Biotin

A column-based approach to antibody biotinylation and clean-up 59.1 Yes No No
Maleimide-PEG2-Biotin

EZ-Link Maleimide PEG2-Biotin

A simple, water-soluble biotinylation reagent specific for sulfhydryls 29.1 Yes No No
Maleimide Solid Phase Biotinylation Kits

EZ-Link Maleimide-PEG Solid-Phase Biotinylation Kit - Prepacked Column

A column-based approach to antibody biotinylation and clean-up N/A N/A N/A N/A
Maleimide Solid Phase Biotinylation Kits

EZ-Link Maleimide-PEG Solid-Phase Biotinylation Kit - Mini-Spin Columns

A column-based approach to antibody biotinylation and clean-up N/A N/A N/A N/A
Pierce Protein Methods Library
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