22341-MS-PEG-4-250

We offer activated linear and branched derivatives of polyethylene glycol (PEG) for pegylation and the PEG-modification of peptides and proteins via primary amines and sulfhydryl groups. These reagents are typically used to increase solubility, prolong stability, and reduce immunogenicity. Certain experimental systems and assay platforms depend on the ability to alter the mass, solubility or other properties of proteins, immunogens, reaction vessels and other materials.

View PEGylation reagents

Amine-reactive PEGylation reagents

Covalent modification with PEG groups requires PEG compounds that contain a reactive or targetable functional group at one end. The simplest method for PEGylating proteins, which are rich in surface primary amines, is to use a PEG compound that contains an NHS ester group at one end.

  • MS(PEG)n reagents are available in PEG lengths (n = 4, 8, 12 and 24).
  • TMS(PEG)n is a branched form of this reagent, containing three methyl-PEG12 arms attached to a PEG4-NHS ester stem.

Sulfhydryl-reactive PEGylation reagents

MM(PEG)n compounds are linear reagents for PEGylating sulfhydryl groups. At the end opposite the inert methyl group(s) is a maleimide moiety, which reacts to form stable thioether bonds with sulfhydryl groups.

  • MM(PEG)n is available in two PEG lengths (n = 12 and 24).

PEGylated amino acids and amine compounds

MA(PEG)n and CA(PEG)n are polyethylene glycol compounds of discrete length (n = 4, 8, 12 and 24) containing methyl-and-amine or carboxyl-and-amine ends. While these functional groups are not spontaneously reactive, they are easily targeted by various crosslinking and immobilization reagents for the construction of surface chemistries.

Choose the right PEGylation reagent for your experiment

PEGylation reagents Product Spacer arm (Å)
Amine-Reactive PEG (Linear)
  • Reactive group: N-hydroxysuccinimide ester
  • Target functional group: Primary amines  
  • Application: PEGylation of a protein or surface, terminating with a methyl group
MS(PEG)4 16.4Å
MS(PEG)8   30.8Å
MS(PEG)12 44.9Å
MS(PEG)24 88.2Å
Amine-reactive PEG (Branched) 
  • Reactive group: N-hydroxysuccinimide ester
  • Target functional group: Primary amines
  • Application: PEGylation of a protein or surface, terminating with a methyl group
TMS(PEG)12 77.5Å
Sulfhydryl-reactive compounds
  • Reactive group: Maleimide
  • Target functional group: Reduced sulfhydryls
  • Application: Branched PEGylation of a protein or surface, terminating with a methyl group
MM(PEG)12 51.9Å
MM(PEG)24 95.3Å
Carboxy-PEG-amine compounds
  • Reactive group: Amine or carboxylic acid
  • Target functional group: N-hydroxysuccinimide, oxidized carbohydrates or amines via EDC
  • Application: PEGylation of a protein or surface, terminating with a carboxylic acid or primary amine
CA(PEG)4 18.1Å
CA(PEG)8 33.6Å
CA(PEG)12 46.8Å
CA(PEG)24 89.8Å
Carboxy-PEG-lipoamide compounds
  • Reactive group: Bidentate thiol
  • Target functional group: Silver, gold and other metal surfaces
  • Application: PEGylation of metal surfaces, terminating with a carboxylic acid group
CL(PEG)12 56Å
Carboxy-PEG-thiol compounds
  • Reactive group: Carboxylic acid or thiol
  • Target functional group: Amines via EDC or Gold surfaces, maleimides, sulfhydryls, haloacetyls
  • Application: PEGylation of a gold or metal surface, terminating with a carboxylic acid
CT(PEG)12 47.8Å
Methyl-PEG-amine compounds
  • Reactive group: Amine
  • Target functional group: N-hydroxysuccinimide, oxidized carbohydrates     
  • Application: PEGylation of a protein, oxidized carbohydrate or surface, terminating with a methyl group
MA(PEG)4 15.5Å
MA(PEG)8 29.7Å
MA(PEG)12 43.9Å
MA(PEG)24 86.1Å
Methyl-PEG-lipoamide compounds
  • Reactive group: Bidentate thiol
  • Target functional group: Silver, gold and other metal surfaces        
  • Application: PEGylation of a gold or metal surface, terminating with a methyl group
ML(PEG)4 23.6Å
Methyl-PEG-thiol compounds
  • Reactive group: Thiol         
  • Target functional group: Gold surfaces, maleimides, sulfhydryls, haloacetyls           
  • Application: PEGylation of a protein or inert material surface, terminating with a methyl group
MT(PEG)4 15.8Å
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