The RED (Rapid Equilibrium Dialysis) and Competition RED systems utilize a disposable device format that is automation-friendly, providing convenience for scientists conducting plasma or tissue-to-plasma protein-binding studies. The inserts are ready to use and do not require any pre-conditioning to the membrane. The single-use format for the RED Device is especially convenient for labs using radioactive materials because the plate can be disposed of easily to help avoid contamination and cleaning. These devices have been validated in assays with high-, medium- and low-protein binding compounds.

  • Disposable tubes—require no presoaking, assembly or specialized equipment
  • Short incubation time—the high membrane surface-to-volume ratio enables equilibrium to be reached in as few as 100 minutes 
  • Flexible—can be used for any number of assays (1–48 assays/plate) and the 96-well footprint is suitable for automated liquid handlers
  • Robust—compartmentalized design helps minimize potential for cross contamination or leakage 
  • Validated—each lot is functionally tested in a protein-binding assay to confirm performance

Choose the right plasma or tissue-to-plasma protein binding product

  Single-Use Red Device, 8K Red Device Inserts, 8K Single-Use Red Device, 12K Competition RED Inserts
Application Plasma protein binding studies Plasma protein binding studies Plasma protein binding studies Drug partioning between plasma and tissue
MWCO 8K

8K

12K 8K
Pre-loaded inserts

 

 
Disposable plate included

 

 
Recommended for variable # of samples per run  

 

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Featured data

Hands-on time of RED Plate
Device Time to reach Equilibrium Leakage Disposable Labor Intensity Automation Accessible Vol. Shift
Multi-Equilibrium
DIALYZER
(Dianorm, Harvard,
Spectrum)
3-4 h Some No ++++ No Minimum
96-Well Equilibrium DIALYZER††
(Harvard Apparatus)
16 h Some Yes +++ Possible Yes
96-Well Micro-Equilibrium
Dialysis
(HTDialysis, LLC)
6 h Some No +++ Possible Yes
RED Device
(Thermo Fisher Scientific)
4-6 h (2 h) None Yes + Yes None

Plasma protein-binding results for seven compounds using the Thermo Scientific RED Device 100-minute protocol are equivalent to results achieved using classic devices found in the literature. A time course study using vortex agitation at 750rpm and 37°C was used to determine the free fraction at equilibration.

Comparison of in-vitro and in-vivo tissue/plasma binding ratios of Taxol in rat.

Comparison of in-vitro and in-vivo tissue/plasma binding ratios of Taxol in rat. Low Taxol presence in brain tissue in vivo indicates the blood-brain barrier inhibits movement of Taxol to the brain.

Video of the RED Device in action

Accessories for RED Device system