Sample preparation remains one of the largest bottlenecks associated with mass spectrometry (MS) analysis, and consistent and reproducible sample preparation can make the difference between a successful analysis and a failed outcome. Thermo Scientific™ reagents and kits are optimized for sample preparation for every step of the process, from depletion strategies to reduce complexity, to protein extraction, enrichment and clean-up for the isolation of low-abundanceproteins. Post digestion, peptide enrichment, fractionation and clean-up steps are often critical for proper analysis. The colorimetric and fluorometric Quantitative Peptide Assays enable sample normalization following digestion.

  • Comprehensive—reagents and kits for every step of the sample preparation workflow, both before and after protein digestion
  • Optimized—all reagents and kits are designed to maximize abundant protein depletion, or to enhance protein and peptide yield
  • Versatile—products available in both complete kits or as standalone reagents
  • Compatible—all kits are designed to be compatible with Mass Spectrometry analysis
  • Validated—all products have been fully tested and processed samples have been analyzed using Thermo Scientific™ Mass Spectrometers

Choose the right mass spec sample prep products for your sample type

  Cultured cells Serum, plasma, or biofluids Tissue Purified protein Intact protein
Abundant protein depletion  

     
Protein enrichment

Protein quantitation assays

Optional

Optional

Gel separation Optional   Optional

 
Protein digestion

Optional
Peptide enrichment / fractionation

   
Peptide cleanup

Optional
Peptide quantitation assays

Products for mass spec sample prep

Kits for albumin, top 2 or top 12 abundant protein depletion to aid in removing the most abundant proteins so that detection of lower abundant proteins is enhanced prior to digestion

The Thermo Scientific™ Pierce™ Mass Spec Sample Prep Kit for Cultured Cells is an easy-to-use, comprehensive kit for preparation of clean peptide mixtures from cultured cells for mass spectrometry (MS) analysis

Protease and phosphatase inhibitors, cocktails and tablets are ideal for protein protection during lysate preparation from tissues and primary or cultured mammalian cells prior to protein digestion

Enrichment techniques include immunoprecipitation, enrichment by PTM (such as phosphoprotein enrichment), or active site labeling and enrichment using activity based probes

A variety of specialty devices and resins that simply and efficiently desalt, buffer exchange and remove detergents from samples

Proteases to break the proteins of interest into smaller fragments, or peptides for mass spec analysis

Successful analysis of low-abundant proteins and identification of post-translationally modified peptides requires multiple steps: enrichment, fractionation and/or clean up

The BCA protein assay is recommended prior to protein digestion to help normalize the protein concentration of samples during each processing step

The new colorimetric and fluorescent peptide quantitation assays help to normalize the peptide concentration of samples during each processing step following protein digestion.

Videos for mass spec sample prep

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