Download ready-to-use protocols to automate KingFisher Instruments.

Thermo Scientific Pierce Magnetic Beads are high-performance, superparamagnetic particles that are designed and validated for use with automated magnetic particle processors, such as the Thermo Scientific KingFisher Flex Magnetic Particle Processor.

The KingFisher Processor runs Thermo Scientific BindIt Software to automate entire procedures. Magnetic beads are incubated, washed and efficiently transferred from well to well according to the procedure to provide reproducible results and excellent recovery. Here we provide optimized BindIt Protocol files to install and direct protein purification procedures on the KingFisher Instrument with Pierce Magnetic Beads.

  1. Download the following file directory (less than 100KB): *** BindIt-Protocols.zip ***
  2. Unzip the file directory on your computer.
  3. Search the table below to identify the individual protocol to install.
  4. In the Home menu of the BindIt Software, click Import on the action panel to open the Import Data dialog box.
  5. Click Browse to select the file (.bdz) that you want to import from a directory.
  6. Select the desired file, and click Open.
  7. Click Next.
  8. Select the location to which you wish to import the protocol. You can also create a new folder, and rename it.
  9. Click Finish. A message appears on the toolbar indicating if the import was successful.

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Magnetic Bead Ligand Application BindIt™ Software Protocol (.bdz file)
Anti-HA Antibody HA-tagged fusion protein purification or immunoprecipitation (IP or Co-IP) BindIt_HA_Tag_Immunoprecipitation.bdz
Anti-c-Myc Antibody Myc-tagged fusion protein purification or immunoprecipitation (IP or Co-IP) BindIt_Myc_Tag_Immunoprecipitation.bdz
Glutathione GST fusion protein purification or pull-down BindIt_GST_Protein_Purification.bdz
Ni-NTA His-tagged fusion protein purification or pull-down BindIt_His_Tag_protein_purification.bdz
NHS Ester Coupling (immobilization) of primary amine molecule (protein, peptide) BindIt_NHS_Coupling.bdz
NHS Ester Affinity purification with coupled (immobilized) primary amine molecule (protein, peptide) BindIt_NHS_IP.bdz
Protein A/G Antibody purification of IgG containing Fc region BindIt_Antibody_Purification.bdz
Protein A Antibody purification of IgG containing Fc region BindIt_Antibody_Purification.bdz
Protein G Antibody purification of IgG containing Fc region BindIt_Antibody_Purification.bdz
Protein L Antibody purification of immunoglobulins containing kappa light chains BindIt_Antibody_Purification.bdz
Protein A/G Immunoprecipitation (IP or Co-IP) BindIt_Protein_AG_Immunoprecipitation.bdz
Protein A Immunoprecipitation (IP or Co-IP) BindIt_Protein_AG_Immunoprecipitation.bdz
Protein G Immunoprecipitation (IP or Co-IP) BindIt_Protein_AG_Immunoprecipitation.bdz
Protein A/G (Classic IP Kit) Immunoprecipitation (IP or Co-IP); kit BindIt_Protein_AG_Immunoprecipitation.bdz
NHS Ester (Direct IP Kit) Immunoprecipitation (IP or Co-IP) or pull-down after coupling (immobilization) of IgG, protein or peptide to beads; kit BindIt_Direct_IP_co-IP.bdz
Protein A/G (Crosslink IP Kit) Immunoprecipitation (IP or Co-IP) after crosslinking IgG to beads; kit BindIt_Crosslink_IP_co-IP.bdz
Protein A/G (Magnetic ChIP Kit) Chromatin immunoprecipition (ChIP) of protein-DNA complexes; kit BindIt_Magnetic_ChIP.bdz
Streptavidin Purification or enrichment of biotinylated or desthiobiotinylated molecules using heated elution BindIt_SA_Immunoprecipitation_heated_elution.bdz
Streptavidin Purification or enrichment of biotinylated or desthiobiotinylated molecules using low-pH elution BindIt_SA_Immunoprecipitation_low_pH_elution.bdz
Titanium Dioxide (TiO2) Phosphopeptide enrichment in deep-well plates (peptides containing phosphorylated amino acids, e.g., phosphoserine, phosphotyrosine or phosphotyrosine) BindIt_Phosphopeptide_enrichment_deepwell_plate.bdz
Titanium Dioxide (TiO2) Phosphopeptide enrichment in PCR plates (peptides containing phosphorylated amino acids, e.g., phosphoserine, phosphotyrosine or phosphotyrosine) BindIt_Phosphopeptide_enrichment_PCR_plate.bdz