YPerScan

Extract total protein from mammalian tissue or cell samples using mild but efficient lysis reagents. These reagents have been optimized and validated with specific tissue types, as well as in primary and cultured mammalian cells. Protein extracts are compatible with most protein assays and typical downstream applications. These reagents offer higher protein yield than other commercially available or homemade formulations.

These convenient single formulation reagents typically allow samples to be processed in 5–10 minutes. When lysing primary and cultured cells, mechanical disruption is not required.

Choose a total protein extraction reagent for your experiments

 
T-PER  Tissue Protein Extraction Reagent N-PER  Neuronal Protein Extraction Reagent M-PER Mammalian Protein Extraction Reagent RIPA Lysis Buffer IP Lysis Buffer
Compatible sample types Heart, liver, kidney, lung, spleen Brain tissue and primary neurons Cultured mammalian and primary cells Cultured mammalian cells Cultured mammalian cells
Sample processing time 10 min 5–10 min 5 min 10 min 10 min
Mechanical disruption required? Yes Yes, for tissue samples No No No
Sample amount 25 g fresh tissue per 500 mL 10 g tissue or 200 x 10 cm dishes of primary cultured neurons per 100 mL 25 g of wet cells (10 million cells per 1 mL of reagent) 5 million cells per 1 mL of reagent 5 million cells per 1 mL of reagent
Protein assay compatibility BCA, Coomassie Plus, Pierce 660, Detergent Compatible Bradford BCA, Pierce 660, Detergent Compatible Bradford BCA, Coomassie Plus, Pierce 660, Detergent Compatible Bradford BCA, Detergent Compatible Bradford (dilute 1:3) BCA, Pierce Detergent Compatible Bradford
Downstream compatibility IP, western blots, ELISA, EMSA, purification, kinase assays, activity assays & amine reactive labeling IP, pulldowns, western blots, ELISA, enzyme assays & amine reactive labeling IP, western blots, ELISA, EMSA, reporter assays, purification, activity assays & amine reactive labeling Western blots, ELISA & purification Western blots, ELISA,
IP & reporter assays
Protease or phosphatase  inhibitors recommended? Yes, both Yes, both Yes, both Yes, both Yes, both
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Additional cell lysis products

Efficiently lyse E.coli and other bacterial cells and gently extract native proteins with these nonionic detergent solutions.

Extract soluble protein from baculovirus-infected insect cells grown in suspension or monolayer culture with this gentle and effective lysis reagent.

Effectively extract protein from all kinds of dry and fresh plant tissue without liquid nitrogen using this plant cell-optimized kit.

Extract and solubilize proteins for analysis from yeast cells with the Y-PER Reagent or the fully dialyzable Y-PER Plus Reagent.

NE-PER_GelShiftAssay450x
Protein yield from various cell types using M-PER Mammalian Protein Extraction Reagent. Cells were harvested at 85% confluency, washed twice and collected in ice-cold PBS and counted. For each cell type, 1 x 10^6 cells were pelleted by centrifugation at 2,000 x g for 5 minutes and lysed in 1 mL M-PER Reagent for 5 minutes. The cell lysates were clarified by centrifugation at 14,000 x g for 10 minutes and the supernatant was collected and the protein concentration (µg/million cells) was determined using the Pierce BCA Protein Assay (Part No. 23227).