These reagent-based kits are optimized for the isolation and enrichment of organelles, including mitochondria, lysosomes, and synaptosomes. Isolated mitochondria, lysosomes and extracted synaptic proteins are compatible with protein assays and typical downstream applications. These reagents provide high protein yield and enable consistent results due to our stringent controls over ingredient quality and formulation.
Select a mitochondria isolation kit for your experiment
|Kit||Mitochondria isolation kit for tissue||Mitochondria isolation kit for cultured cells|
|Compatible sample types||Heart and liver tissue||Cultured mammalian cells|
|Sample processing time||1 hr||40 min|
|Mechanical disruption required?||Dounce optional||Dounce optional|
|Amount of sample processed||50 samples each containing 50 to 200 mg of soft or hard tissue||50 samples each containing 20 million cultured mammalian cells|
|Protein assay compatibility||BCA, Coomassie Plus, Pierce 660, Detergent Compatible Bradford||BCA, Pierce 660, Detergent Compatible Bradford|
|Downstream compatibility||Western blot, ELISA, amine reactive labeling, apoptosis, signal transduction and metabolic studies||Western blot, ELISA, amine reactive labeling, apoptosis, signal transduction and metabolic studies|
|Protease and/or phosphatase inhibitors recommended?||Yes, both||Yes, both|
|Order now||Order now|
Retain activity while enriching key subcellular components
Integrity of mitochondria extracted from cultured cells. C6 cells were extracted with Thermo Scientific Mitochondria Isolation Kit using the reagent-based method (A and B) or Dounce homogenization (C and D). Mitochondrial (M) and cytosolic (C) fractions were analyzed via western blot for cytochrome C (A and C) or voltage-dependent anion channel (VDAC) (B and D). Thermo Scientific SuperSignal West Pico Chemiluminescent Substrate (Part No. 34080) was used for detection. Results indicate that mitochondria remained intact.
Greater enrichment of synaptic proteins is achieved in samples prepared using the Thermo Scientific Syn-PER Reagent than with a homebrew reagent. Total protein (10 µg) from mouse brain tissue homogenates (H), cytosol (C) fraction, and synaptosome suspension (Syn) were analyzed by western blot. The total protein yield in the synaptosome suspension produced from fresh mouse brain (200 mg) was three-fold greater using the Syn-PER Reagent compared to a typical homebrew reagent.
For Research Use Only. Not for use in diagnostic procedures.