89839-Lyso-Enrichment-Kit-230

These reagent-based kits are optimized for the isolation and enrichment of organelles, including mitochondria, lysosomes, and synaptosomes. Isolated mitochondria, lysosomes and extracted synaptic proteins are compatible with protein assays and typical downstream applications. These reagents provide high protein yield and enable consistent results due to our stringent controls over ingredient quality and formulation.

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89839-Lyso-Enrichment-Kit-210

Select a mitochondria isolation kit for your experiment

Kit Mitochondria isolation kit for tissue Mitochondria isolation kit for cultured cells
Compatible sample types Heart and liver tissue Cultured mammalian cells
Organelle isolated Mitochondria Mitochondria
Sample processing time 1 hr 40 min
Mechanical disruption required? Dounce optional Dounce optional
Amount of sample processed 50 samples each containing 50 to 200 mg of soft or hard tissue 50 samples each containing 20 million cultured mammalian cells
Protein assay compatibility BCA, Coomassie Plus, Pierce 660, Detergent Compatible Bradford BCA, Pierce 660, Detergent Compatible Bradford
Downstream compatibility Western blot, ELISA, amine reactive labeling, apoptosis, signal transduction and metabolic studies Western blot, ELISA, amine reactive labeling, apoptosis, signal transduction and metabolic studies
Protease and/or phosphatase inhibitors recommended? Yes, both Yes, both
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Retain activity while enriching key subcellular components

Integrity of mitochondria extracted from cultured cells. C6 cells were extracted with Thermo Scientific Mitochondria Isolation Kit using the reagent-based method (A and B) or Dounce homogenization (C and D). Mitochondrial (M) and cytosolic (C) fractions were analyzed via western blot for cytochrome C (A and C) or voltage-dependent anion channel (VDAC) (B and D). Thermo Scientific SuperSignal West Pico Chemiluminescent Substrate (Part No. 34080) was used for detection. Results indicate that mitochondria remained intact.

Greater enrichment of synaptic proteins is achieved in samples prepared using the Thermo Scientific Syn-PER Reagent than with a homebrew reagent. Total protein (10 µg) from mouse brain tissue homogenates (H), cytosol (C) fraction, and synaptosome suspension (Syn) were analyzed by western blot. The total protein yield in the synaptosome suspension produced from fresh mouse brain (200 mg) was three-fold greater using the Syn-PER Reagent compared to a typical homebrew reagent.