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Thermo Scientific™ dialysis units help facilitate the rapid and trouble-free dialysis of sample volumes from 10 μL to 250 mL. Unlike standard flat tubing, these innovative devices do not require knots or clips that can lead to leaking and sample loss.

Pierce™ 96-well Microdialysis Plates and Slide-A-Lyzer™ Dialysis MINI Devices are ideal for small volumes, Slide-A-Lyzer™ Dialysis Cassettes (original and G2) are recommended for small to medium volumes, and Slide-A-Lyzer™ Dialysis Flasks are recommended for larger volumes. 

  • Excellent sample recoveries—low-binding plastic and membrane help minimize sample loss compared to filtration and resin systems
  • Convenient—easy-to-grip format helps simplify sample addition and removal with syringe and/or pipette
  • Secure—sealed membranes help prevent leakage that can occur with dialysis tubing and homemade devices
  • Validated—each device is leak-tested during production

Choose the right dialysis device for your experiments

 
  Pierce™ 96-well Microdialysis Plates Slide-A-Lyzer™ MINI Dialysis Devices Slide-A-Lyzer™ Dialysis Cassettes (original) Slide-A-Lyzer™ G2 Dialysis Cassettes Slide-A-Lyzer™ Dialysis Flasks
External float required? No No Yes No Optional
Method of sample addition/removal Pipette Pipette Syringe Pipette or syringe Pipette
Volume range 10–100 μL 10 μL–2 mL 0.1–30 mL 0.25–70 mL 150–250 mL
Number of devices with different volume capacities 1 3 4 5 1
Molecular weight cutoffs (MWCOs) 3.5K, 10K 2K (0.1 mL size only), 3.5K, 10K, 20K 2K, 3.5K, 7K (0.1 and 0.5 mL only), 10K, 20K 2K, 3.5K, 7K (0.1 and 0.5 mL only), 10K, 20K 2K, 3.5K, 10K, 20K
Automation compatible? Yes No No No No
Integrated buffer reservoir? Yes Yes, except 0.1 mL size No No No
Gamma-irradiated options? No No Yes, 10K only Yes, 10K only No
Molecular weight cutoff (MWCO) color coded? No Yes Yes Yes Yes
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Learn more about our dialysis products

2K MWCO

2K_MWCO_graph
Sample retention by the 2K MWCO Thermo Scientific Slide-A-Lyzer Cassette membrane. Vitamin B12, bacitracin, tyrosine kinase peptide 1, biotin-TPKs substrate, protein kinase Ce (PKCe) peptide substrate and insulin A chain model systems (0.5-1 mg/mL) in either saline or 0.2M carbonate bicarbonate buffer, pH 9.4 were dialyzed overnight (17 hours) at 4°C. The amount of retentate was estimated using either the Thermo Scientific™ Pierce™ BCA Protein Assay or absorption at 360 nm (for vitamin B12).

3.5K MWCO

3-5K_MWCO_graph
Sample retention by the 3.5K MWCO Thermo Scientific Slide-A-Lyzer Cassette membrane. Vitamin B12, insulin B chain and insulin (1 mg/mL) in either saline or 0.2M carbonate bicarbonate buffer, pH 9.4 were dialyzed overnight (17 hours) at 4°C. The amount of retentate was estimated using either the Pierce BCA Protein Assay or absorption at 360 nm (for vitamin B12).

7K MWCO

7K_MWCO_graph
Sample retention by the 7K MWCO Thermo Scientific Slide-A-Lyzer Cassette membrane. Vitamin B12, insulin B chain, insulin and cytochrome C(1 mg/mL) in either 0.15M sodium chloride or 0.2M carbonate bicarbonate buffer, pH 9.4 were dialyzed overnight (17 hours) at 4°C. The amount of retentate was estimated using either the Pierce BCA Protein Assay or absorption at 360 nm (for vitamin B12).

10K MWCO

10K_MWCO_graph
Sample retention by the 10K MWCO Thermo Scientific Slide-A-Lyzer Cassette membrane. Insulin B chain, insulin and cytochrome C (1 mg/mL) in either 0.15M sodium chloride or 0.2M carbonate bicarbonate buffer, pH 9.4 were dialyzed overnight (17 hours) at 4°C. The amount of retentate was estimated using the Pierce BCA Protein Assay.

20K MWCO

20K_MWCO_graph
Sample retention by the 20K MWCO Thermo Scientific Slide-A-Lyzer Cassette membrane. Insulin B chain, cytochrome C, lysozyme and myoglobin were dialyzed overnight (17 hours) at 4°C in PBS, pH 7.4. The amount of retentate was estimated using the Pierce BCA Protein Assay.

Thermo Scientific™ Slide-A-Lyzer™ Cassettes protect your precious samples during dialysis and help minimize the hassles and risks of losing your samples using slippery tubing. They're available in sizes from 10 µL to 250 mL and in a full range of MWCOs.

 
Traditional tubing vs. Cassette
Slippery when wet
Flat tubing is difficult to handle when wet, increasing risk of sample loss during sample addition/removal
sample handling Easy handling and secure sample delivery
Plastic frame and cap allow for secure sample addition/removal
Risk of sample loss
Sample can leak out if clamp becomes loose or falls off
sample recovery Sample protection
Welded membrane and leak-proof cap helps minimize the risk of sample loss
Time consuming
Non-optimized design that typically requires overnight dialysis
speed Fast and efficient
Innovative design with high surface/volume ratio allows for more rapid dialysis than conventional tubing
Comparison of Thermo Scientific Slide-A-Lyzer Cassettes with traditional dialysis tubing.

Thermo Scientific Slide-A-Lyzer Flasks Video

Other featured dialysis products

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Snakeskin™ Dialysis Tubing

A ready-to-use form of traditional dialysis membrane tubing for 10 to 100 mL samples that does not require presoaking or boiling prior to use. SnakeSkin™ Dialysis Tubing is composed of regenerated cellulose and is available in 3 molecular weight cutoffs and 3 internal diameters.

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