PB2260_slidealyzer_dialysis_cassettes-230

Slide-A-Lyzer™ Dialysis Cassettes help facilitate the rapid and effective dialysis of sample volumes ranging from 100 μL to 30 mL. The cassette design helps maximize surface area to sample volume ratio and enables excellent sample recoveries.

In comparison to standard flat tubing, these innovative cassettes do not require knots or clips that may lead to leaking and sample loss, helping to obtain more complete sample recovery. They are available in 4 volume capacities and in molecular weight cutoffs (MWCOs) of 2K, 3.5K, 7K, 10K, and 20K.

Choose your Slide-A-Lyzer™ cassette by volume & molecular weight cutoff (MWCO)

  Volume range
Molecular weight cut off 0.1–0.5 mL 0.5–3 mL 3–12 mL 12–30 mL
2K Catalog number
66205
Catalog number
66203
Catalog number
66212
Catalog number
66230
3.5K Catalog number
66333
66335 (kit)
Catalog number
66330
66332 (kit)
Catalog number
66110
66107 (kit)
Catalog number
66130
7K Catalog number
66373
Catalog number
66370
66372 (kit)
Catalog number
66710
66707 (kit)
Not available
10K Catalog numbers
66383, 66384
66385 (kit)
Catalog numbers
66380, 66381
66382 (kit)
Catalog numbers
66810, 66811
66807 (kit)
Catalog number
66830
10K
(gamma irradiated)
Catalog number
66454
Catalog number
66455
Catalog number
66456
Catalog number
66457
20K Catalog number
66005
Catalog number
66003
Catalog number
66012
Catalog number
66030

Learn more about Slide-A-Lyzer™ Cassettes

Table 1. Quantitative sample recovery.

Three sample volume batches of water (0.5 mL, 1.7 mL and 3.0 mL) were loaded and recovered per the respective manufacturer’s instructions in a Slide-A-Lyzer Dialysis Cassette or conventional dialysis tubing to determine the volumes of recovery. Water volume recovered was determined gravimetrically.

Sample volume loaded Thermo Scientific
Slide-A-Lyzer Dialysis Cassette
% volume recovery
Traditional dialysis tubing
% volume recovery
3.0 mL 99.47 92.32
1.7 mL 99.30 93.12
0.5 mL 98.76 887.51

Thermo Scientific Slide-A-Lyzer Cassette procedure summary

PB2260_slide-a-lyzer_cassette_procedure1

1. Insert syringe needle through the gasket via one of the corner ports. Inject the sample, withdraw the excess air and remove the syringe.

PB2260_slide-a-lyzer_cassette_procedure2

2. Attach a float buoy and dialyze. (Buoys also serve as convenient bench-top stands for the cassettes.)

PB2260_slide-a-lyzer_cassette_procedure3

3. Insert empty syringe needle at a second corner port. Inject air to expand the cassette chamber, then withdraw the dialyzed sample.

Thermo Scientific Slide-A-Lyzer™ Dialysis Cassette Video

Western Done
ibind-banner-362x140