PB2200_zeba_desalting_products

Zeba™ desalting products contain proprietary high-performance resins with exceptional desalting and protein-recovery characteristics. They can help process even very dilute protein samples, with high levels of protein recovery and greater than 95% retention (removal) of salts and other small molecules.

  • High performance—proprietary resin enables excellent protein recovery and efficient contaminant removal
  • Flexible—available in spin columns, filter spin plates, and cartridges for a range of needs
  • Fast—no fraction screening or waiting for protein to emerge by gravity flow
  • Economical—cost-effective products that offer great performance

Choose the right Zeba™ desalting device for you

 
  Zeba™ Spin Desalting Columns Zeba™ 96-well Spin Plates Zeba™ Chromatography Columns
Sample volume range 2 μL–4 mL 20–100 μL 15–250 μL or
100–1500 μL
Bed volume range 75 μL–10 mL 550 μL 1 mL or
5 mL
Number of devices with different volume capacities 5 1 2
Molecular weight cutoffs (MWCOs) 7K, 40K 7K, 40K 7K
Automation compatible? No Yes No
FPLC compatible? No No Yes
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Compare Zeba Spin Desalting Column performance

Figure 1. Better performance with Thermo Scientific Zeba Spin Desalting Columns. Zeba Spin Desalting Columns provide excellent protein recovery while providing minimal sample dilution over a wider range of sample concentrations and volumes compared to alternative products. Zeba Spin Desalting Columns, 0.5 mL (7K MWCO)(Product # 89882), GE SpinTrap G-25 and Bio-Rad Micro BioSpin 6 spin columns were used to desalt 40, 80 and 120 μL samples of a HeLa lysate at a concentration of 0.2 and 1 mg/mL. Desalting was performed according to the manufacturers’ recommended protocols. Protein recovery was analyzed by SDS-PAGE. For each electrophoresis gel, an aliquot of starting sample equal to 1 μg of HeLa lysate was loaded in lane 1 as the load control; all other desalted samples were loaded in the gel at the same volume as the load control. Differences in intensity between lanes are a combination of protein recovery and sample dilution caused by desalting.

Table 1. Comparison of protein recovery, from the 7K MWCO and 40K MWCO, 0.5 mL Thermo Scientific Zeba Spin Desalting Columns.

  Typical % recovered
from 100 μL sample
Recovery of Concentration loaded Size of molecule 7K Zeba Column 40K Zeba Column
Ubiquitin 0.5 mg/mL 8.7 kDa 75% 60%
α-Lactalbumin 1.0 mg/mL 14.1 kDa 85% 75%
Soybean trypsin
inhibitor
0.5 mg/mL 20.1 kDa 85% 65%
Carbonic anhydrase 0.5 mg/mL 29 kDa 90% 75%
Ovalbumin 0.5 mg/mL 44 kDa 90% 85%
Bovine serum
albumin
0.5 mg/mL 66 kDa >90% >90%
Human IgG 0.5 mg/mL 150 kDa >90% >90%

Table 2. Comparison of small molecule removal, for the 7K MWCO and 40K MWCO, 0.5 mL Thermo Scientific Zeba Spin Desalting Columns.

  Typical % recovered
from 100 μL sample
Removal of Concentration loaded Size of molecule 7K Zeba Column 40K Zeba Column
NaCl 1 M 58.44 Da >99% >99%
Dithiothreitol 0.5 M 154 Da 99% 99%
Sulfo-NHS-LC-biotin 0.27 mM 557 Da 85% 85%
Dy549 dye 0.2 mM 1026 Da 75% 90%
Bacitracin 0.5 mg/mL 1200 Da 75% 95%
Vitamin B12 0.5 mg/mL 1386 Da 85% 95%

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