88310-88312-Kinase-Kit-230

Numerous techniques are available for the isolation of cellular proteins based on their post-translational modification (PTM) or active binding site. Protein enrichment is essential for isolating low-abundant proteins or isolating protein classes. Enrichment of specific proteins or protein complexes can most easily be accomplished using target-specific immobilized antibody or PTM specific affinity binding. The novel Thermo Scientific™ ActivX™ probes and enrichment kits enable the specific targeting of several enzyme classes, including kinases, GTPases, and serine hydrolases.

  • Comprehensive—reagents and kits for multiple protein enrichment strategies
  • Optimized—all reagents and kits are designed to maximize protein yield and reduce nonspecific binding
  • Convenient—easy-to-use spin formats enable more rapid enrichment of proteins
  • Flexible—kits can be used with either cultured mammalian cells or tissues

Choose the right protein enrichment kit for your application

 
  Phospho-
protein Enrichment Kit
Glycoprotein Enrichment Kit, ConA Glycoprotein Enrichment Kit, WGA Ubiqitin Enrichment Kit Kinase Enrichment Kit with ATP Probe ActivX™ TAMRA-FP Serine Hydrolase Probe
Target Phosphorylated proteins α-linked mannose and terminal glucose residues N-acetyl glucosamine (GlcNAc) Polyubiquin-ated proteins ATP binding sites Active serine hydrolase enzymes
Binding/
labeling mechanism
Metal chelate affinity to phosphate groups Lectin affinity to sugars Lectin affinity to sugars Antibody affinity to target Biotinylated ATP analog  to active-site lysine TAMRA-labeled fluoro
phosphanate binds to active site serine
Processing time 2 hr <1 hr <1 hr <45 min 6.5 hr 3 hr
Enrichment format IMAC-agarose Immobilized conA-lectin-agarose Immobilized WGA-lectin-agarose Immobilized polyubiqitin affinity-agarose Immobilized strepavidin-agarose Immobilized anti-TAMRA mAb-agarose
  90003
(10 columns)
89804 (10 rxn) 89805 (10 rxn) 89899 (15 rxn) 88310 (16 rxn) 88318 (6.8 µg)

For downstream mass spectrometry analysis, peptide enrichment and/or fractionation is often necessary to detect peptides from low-abundance proteins and/or for reducing sample complexity for proteomic analysis.

Faster and higher phosphoprotein yields with the Pierce® Phosphoprotein Enrichment Kit.

Kit Phosphoprotein yield (ug)* Enrichment time (hours)
Pierce® Phosphoprotein Enrichment Kit 300 1.5
Supplier Q kit 88 4.5
Supplier I kit 52** 3.5
Supplier C kit 160 3
Supplier E kit Too dilute to determine 5
*From 2 mg total protein.
**Based on maximum 1 mg load per manufacturer’s protocol.