44894-AminoLink-Plus-230

We offer a variety of Thermo Scientific™ activated supports and accessories for the immobilization of proteins, antibodies, and other molecules. These resins are available separately or in convenient kits.

  • Product choice—a variety of coupling chemistries  to meet different immobilization strategies
  • High performance—resins that maximize coupling efficiency and binding capacity
  • Flexible—available in multiple pack sizes and formats

Choose the right immobilization solution for your experiment

 
  Pierce® NHS-Activated Agarose AminoLink Coupling Resin AminoLink Plus Coupling Resin SulfoLink Coupling Resin GlycoLink Coupling Resin CarboxyLink Coupling
Target -NH2 -NH2 -NH2 -SH -CHO -COOH
Support 6% agarose (slurry or dry powder) 4% agarose 4% agarose, highly crosslinked 6% agarose Ultralink (polyacrylamide/
azlactone copolymer)
4% agarose
Coupling capacity >25 mg/mL resin 1–20 mg/mL resin 20 mg/mL resin 20 mg/mL resin 1–10 mg/mL resin 5 mg/mL resin
Coupling
time
30 min 4 hr 4 hr 2–3.5 hr 30 min 4 hr
Recommended for Proteins Proteins, antibodies Proteins, antibodies Proteins, peptides, antibodies Glycoproteins, polyclonal antibodies Unmodified peptides
Advantages High coupling efficiency and capacity High coupling efficiency High coupling efficiency Allows for gentle elution Correctly orients antibodies during coupling Flexible and gentle coupling conditions
Disadvantages Narrow pH range (7–9) May be coupled at antigen-binding site May be coupled at antigen-binding site Protein must be reduced first Not recommended for monoclonal antibodies Not recommended for antibodies
  26196, 26197 (dry resin),
 26200 (slurry)
20381 (10 mL), 20382 (50 mL) 20501 (10 mL), 20505 (50 mL) 20401 (10 mL), 20402 (50 mL), 20404 (250 mL) 53149 (10 mL) 20266 (25 mL)
Coupling-Efficiency-Graph
|Thermo Scientific Pierce NHS-Activated Agarose more effectively couples dilute samples than competing products.| Rabbit IgG (0.5 mg) was applied to 0.25 mL of activated agarose in PBS at pH 7.2 for 30 minutes. The protein concentration of the flow-through and washes (uncoupled IgG) was determined using the Pierce 660nm Protein Assay. The bound IgG was calculated by subtracting the amount of uncoupled IgG from the amount added. The coupling efficiencies of both dry and slurry forms of Pierce NHS-Activated Agarose were higher than NHS-activated Sepharose™ 4 Fast Flow (GE Healthcare) and Affi-Gel™ 10 Gel (Bio-Rad).
44999-002-SulfoLink-Peptide-TCEP
|Figure 1. TCEP effectively reduces peptide sulfhydryls (cysteine residues) to maximize covalent immobilization efficiency to SulfoLink Coupling Resin.| Peptide A (0.5 mg) and human calcitonin peptide (1 mg) were incubated with (+) or without (-) 25 mM TCEP for 30 minutes and then reacted to 2 mL of SulfoLink Resin for 30 minutes using the default kit procedure. Each peptide contained a C-terminal cysteine, as well as an N-terminal fluorescent tag by which the proportion of non-coupled peptide was determined upon recovery of the reaction solution.

High-yield protein coupling

Immobilization efficiencies for various proteins on 1 mL of AminoLink Plus Resin when immobilized using the pH 7.2 coupling procedure.

Protein Protein (mg) reacted Protein (mg) coupled Coupling efficiency (%)
Protein G 4.6 4.0 83
Mouse IgG 4.7 4.5 96
Rat IgG 4.7 4.4 93
GAM*-IgG 0.9 0.8 84
Human IgG 4.8 4.6 97
Human IgM 0.9 0.8 93
*GAM-IgG is goat anti-mouse IgG, a secondary antibody.

Additional activated supports and accessories are available, as well as convenient kit formats that included disposable columns, buffers, and coupling reagents. Some activated supports are also available in magnetic bead formats.