The proteins should be arrayed in 96 well plates. Send 50%micro;l of each protein at concentration 0.1-1mg/ml. In addition, please include a 50µl aliquot of your sample buffer. Ship on dry ice.
8) Proteins derived from Invitrogen’s Ultimate™ human ORF collection
Please submit an excel spreadsheet that includes a list of desired human Ultimate™ ORF clones with accession numbers. These can be found on Invitrogen’s web-site by searching the human Ultimate™ ORF Browser.
- Requested proteins should be submitted as excel spreadsheets in increments of 92 (discounts for partial plates <92 clones are not available).
Some of the proteins chosen may fail to express. We guarantee that 50% of the proteins will be detectable by Western blot analysis at the expected size or greater and print on the slide. If the success rate falls under 50%, the run will be repeated. Invitrogen may request alternative Ultimate™ ORF clones be chosen.
Please note: the proteins may express in high enough quantities to be printed but fail to be detectable on the Western blot control. If the expression run success rate is greater than 50% all 92 proteins/per submitted plate will be printed on the arrays.