10X the protein yield from your T7 expression

MagicMedia™ E. coli Expression Medium

  • 3-10 times higher protein yields with much less hands-on time
  • Simple protocol eliminates tedious O.D. monitoring and induction steps
  • Compatible formulation works with all T7-regulated E. coli vectors and BL21 and derivative strains

Due to their ease of use, relatively high yields, and simple scalability, T7-regulated E. coli expression systems and BL21 hosts are often chosen for generating recombinant protein. Now you can further increase yields and make this already easy expression system even easier-simply by switching to Invitrogen's MagicMedia™ E. coli Expression Medium.

Superior protein yields

The MagicMedia™ formulation provides optimal conditions for effectively regulating expression of the T7 promoter used in many E. coli systems. This routinely results in high-density cultures that produce high recombinant protein yields that exceed those achieved using LB medium with IPTG inducer (Figure 1).

Increase MagicMedia Yields

Figure 1.  Increase protein yields with the MagicMedia™ E. coli Medium.

Eight different human ORFs were cloned into the Champion™ pET300/NT-DEST vector using Gateway® cloning. Positive clones were transformed into BL21(DE3) E. coli. The clones were expressed in LB + IPTG (lane 1), ready-to-use liquid MagicMedia™ Medium (lane 2), or MagicMedia™ prepared from powder (SoluPouch™ format) (lane 3). Two hundred microliters of each culture was lysed and analyzed on a Coomassie®-stained NuPAGE® Novex® 4–12% Bis-Tris Gel. M: SeeBlue® Protein Standard

Ultimate convenience

MagicMedia™ E. coli Expression Medium is extremely easy-to-use and does not require the time-consuming Optical Density (O.D.) monitoring and induction steps as conventional LB + IPTG methods. Simply inoculate prepared MagicMedia™ with the appropriate seed culture, grow the culture overnight, and harvest (Figure 2). In addition, the E. coli culture volume required when using the MagicMedia™ formulation is significantly reduced compared to LB volumes, simplifying handling and scale-up processes. MagicMedia™ Medium is also ideal for efficiently performing simultaneous expression of many clones for high-throughput applications.

Effortless MagicMedia Proto

Figure 2.  Effortless MagicMedia™ protocol.

Two all-inclusive formats: powder and liquid

MagicMedia™ E. coli Expression Medium consists of proprietary components A and B and is available in two convenient formats to meet specific needs-premixed powder packaged in a convenient, water-dissolvable bag (SoluPouch™ format) or a ready-to-use, presterilized liquid. For the SoluPouch™ format, simply place the entire pouch containing the component A powder in water and autoclave. The SoluPouch™ and powder will dissolve completely-the pouch will not have any affect on the cells you culture. Then add the liquid component B and your antibiotic. There's no weighing out materials, no mess, and no additional reagents needed (except your selection agent). For the ready-to-go liquid format, simply combine components A and B and add your antibiotic-no autoclaving is necessary. With either format, you'll see how easy it is to incorporate MagicMedia™ Medium into your current E. coli expression procedures.