Nitrocellulose membrane is a high quality membrane ideal for blotting of proteins and nucleic acids. The major features of the nitrocellulose membrane available from Invitrogen include:

  • Composed of 100% pure nitrocellulose to provide high-quality transfer
  • Contains no support fabric or detergents
  • Compatible with commonly used transfer conditions and detection methods such as staining, immunodetection, fluorescence, or radiolabeling
  • Provides high-sensitivity with low background
  • Supplied in a pre-cut, pre-assembled membrane/filter paper sandwich for convenience

Stripping and reprobing of nitrocellulose membranes is not recommended. For experiments requiring re-use please see our PVDF membranes.

Figure 1. Nitrocellulose blot of E-PAGE™ Gel developed with WesternBreeze® Kit using Nitrocellulose Membranes from Invitrogen. MagicMark™ XP Western Protein Standard (5 µL) was loaded into each well of an E-PAGE™ 48 8% Gel and transferred to a pre-cut 0.2 µm nitrocellulose membrane. The blot was probed with 1:1000 Anti-Xpress™ Antibody and detected using the WesternBreeze® Chemiluminescent (Panel A) or WesternBreeze® Chromogenic (Panel B) Immunodetection kit (anti-mouse).