Rapid resequencing of mitochondrial genomes

Mitochondrial DNA sequencing is a useful tool for researchers studying human diseases such as diabetes, certain cancers, and mechanisms of aging. Mitochondrial DNA sequencing is also used in population genetics and biodiversity assessments. Targeted mitochondrial DNA sequencing can be used to detect mutations present in some copies of the mitochondrial genome (heteroplasmic mutations). Finally, mitochondrial DNA sequencing is important for human identification and forensics applications.

Rapid resequencing of mitochondrial genomes for disease research and biodiversity assessment is accomplished using Ion Torrent™ next-generation sequencing.

Mitochodrial sequencing using Ion Torrent™ next-generation sequencing enables:

  • Capacity of 16 samples per run with barcoding
  • Accurate variant calling, especially in hypervariable regions of mitochondria

Highly targeted mitochondrial DNA sequencing is performed by gold-standard Sanger sequencing, using Applied Biosystems® genetic analyzers. The simple workflow can be performed in less than 5 hours using the Applied Biosystems® 3500 Genetic Analyzer.

Step-by-Step Guide to Mitochondrial Sanger Sequencing Products

DNA Purification kit

DNA extraction is a critical first step in the experimental workflow of DNA Sequencing and Fragment analysis. The overall quality, accuracy and length of the DNA sequence read can be significantly affected by characteristics of the sample itself, and the method chosen for nucleic acid extraction. Ideal methods will vary depending on the source or tissue type, how it was obtained from its source, and how the sample was handled or stored prior to extraction.

Recommended Products:

PCR Kit

BigDye® Direct Cycle Sequencing Kit contains reagents to perform the PCR, PCR clean up and sequencing reactions.

Recommended Products:

DNA strands

BigDye® Direct Cycle Sequencing Kit contains reagents to perform the PCR, PCR clean up and sequencing reactions. PCR Clean up and sequencing reaction are perform in a single step.

Recommended Products:

BigDye® Direct cycle sequencing kits

Sequencing Reaction Cleanup

After the sequencing reaction, it is important to remove unincorporated dye terminators and salts that may compete for capillary electrophoretic injection. Unincorporated terminators can co-migrate with the sequencing template, resulting in basecalling er

Recommended Products:

Which Sample Cleanup Product Is Right for You?

Cleanup Method Description & Application Product
Reagent-Based Sequencing Template CleanupScavenges all unincorporated BigDye® terminators. Stabilizes samples before analysis. Best for long and short fragment recovery.
Capillary Electrophoresis

During capillary electrophoresis, the products of the PCR are injected electrokinetically into capillaries filled with polymer. High voltage is applied so that the fluorescent DNA fragments are separated by size and are detected by a laser/camera system.

Which Electrophoresis Instrument (Genetic Analyzer) Is Right for You?

  Instrument
 3730xl
3730xL
3730
3730
3500xl
3500xL
3500
3500
3130xl
3130xL
3130
3130
310
310
Number of Capillaries96482481641
Compatible Applications:
(S) Supported; (A) AB Demonstrated; (C) Customer Demonstrated; (N) Not Supported
mtDNA Sequencing SSSSSSS

Analyze the mitochondrial sequence.

Which Data Analysis Software Is Right for You?

For Research Use Only. Not for use in diagnostic procedures.