FAST AND SIMPLE

TARGET SELECTION
TECHNOLOGY

Transform your research with ready-to-use, custom, and community panels.


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Simplicity

  • 10 ng of DNA per pool
  • FFPE-compatible
  • PCR-based target selection

Scalability

  • Up to 6,144 primers per pool
  • One to hundreds of genes
  • 96 barcodes for multiplexing

Speed

  • Single day from DNA to results
  • 2 hours to design custom panels
  • 3.5 hours for target selection & library preparation

The most comprehensive gene coverage with the least amount of DNA

Ion AmpliSeq™ technology delivers simple and fast library construction for affordable targeted sequencing of specific human genes or genomic regions. Based on ultrahigh-multiplex PCR, Ion AmpliSeq™ technology requires as little as 10 ng of input DNA to target sets of genes, making sequencing of FFPE samples routine on Ion PGM™ Systems.

Alternative target selection methods are lengthy and complex and require large amounts of DNA, making them impractical for sequencing DNA from the formalin-fixed, paraffin-embedded (FFPE) tissues that are commonly used in translational and clinical research. Using just 10 ng of FFPE DNA and one pool of 6,144 primer pairs, variants can be identified in a single day using the Ion AmpliSeq™ Custom workflow. By contrast, the same project using alternative workflows requires 100 times as much DNA, 8 times the pools, and twice the time.

Ion TargetSeq™ Exome Kits offer a simple and flexible workflow that provides single tube enrichment using solution-phase high-density DNA probe capture technology for highly specific enrichment of exons and other targeted regions within the human genome. Ion TargetSeq™ Exome Kits utilize the highest capture probe density (> 2M biotinylated capture probes) along with a probe tiling strategy that provides exceptional capture efficiency resulting in high “on target” sequencing read percentages. The kits combine the advantages of low DNA input, as little as 125 ng of each individual library, with speed - 2.5 day library preparation and capture – that facilitate sample multiplexing with up four barcoded samples in a single target enrichment reaction. The capture protocol and capture probe content have been thoroughly optimized to give superior performance on both Ion Torrent PGM and Proton sequencing instruments. Further, the exome sequencing workflow leverages the enzymatic sheering protocol of Ion Express™ Plus Fragment Library kit for integrated workflow that permits easy automation.

Ion AmpliSeq™ Single Day Workflow

1 Construct Library

Ion AmpliSeq™ Primer pool

2 Prepare Template

Ion OneTouch™ System

3 Run Sequence

Ion PGM™ Sequencer

4 Analyze Data

Torrent Suite™ Analysis Software

5 Annotate Results

Ion Reporter™ Software

For Research Use Only. Not intended for any animal or human therapeutic or diagnostic use.