High Coverage Gene Expression Profiling - HiCEP
The HiCEP method is an amplified fragment length polymorphism (AFLP)-based gene expression profiling method that requires no prior sequence information and has a reduced rate of false positives and a high degree of detection of both coding and non-coding transcripts.
Whole transcriptome expression profiling is historically performed using hybridization-based microarray methods. There are a number of limitations to microarray-based approaches such as low sensitivity and specificity, poor dynamic range, as well as a need for prior sequence information. The HiCEP method was developed to address the shortcomings in gene expression profiling and provide a sensitive method for detecting a large proportion of transcripts in both known and unknown genes, with a low false positive rate.
Step-by-Step Guide to High Coverage Gene Expression Profiling
Add aliquot of PCR product to size standard and Hi-Di Formamide mix; heat denature and proceed with electrophoresis.
Recommended Products: Prepare Sample for Analysis
To learn more about our GeneScan Size standards used in sizing experiments please see our table below.