Small RNA & miRNA Sequencing by Ion Torrent Next-Generation Sequencing
Discover and profile expression of small RNAs
Cutting-edge Ambion® RNA technology, combined with accessible Ion Torrent™ next-generation sequencing, enables any lab to see and discover more with small RNA sequencing.
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Read more about the basics of small RNA sequencing and solutions for the Ion Proton™ and PGM™ Systems
Learn about small RNA sequencing informatics solutions for the fastest way to get to your biological results
Learn about small RNA sequencing solutions
Solutions for small RNA sequencing
Ion PGM™ System with Ion 314™, Ion 316™, and Ion 318™ Chips
Ion Total RNA-Seq Kit v2 to purify small RNA and construct strand-specific libraries
PureLink® miRNA Isolation Kit fast, flexible, and high-quality sequencing of small RNA
Small RNA sequencing workflow for Ion Torrent™ next-generation sequencing systems
Small RNA sequencing Informatics solutions
Ion Torrent™ semiconductor sequencing combines a simple, integrated wet-lab workflow with Torrent Suite™ Software and third-party solutions for fast identification, characterization, and reporting of small RNA expression.
Torrent Suite™ Software automatically provides the sequence reads in exportable BAM files, which can be uploaded to third-party analysis software. Post-run sequencing data are available for download with a simple right-click. Reports are also easily browsed, with expandable analysis plots and straightforward tables summarizing key results to help ensure high-quality sequencing runs.
Third-party software, such as Partek® Flow® software packages, performs read alignment, microRNA level abundance measurements, and multi-sample differential microRNA expression analysis. Additionally, it provides quality-control metrics and interactive data visualization.
Introduction to small RNA sequencing
Since the discovery of microRNA, the study of RNA transcription and regulation of gene expression has grown tremendously through the use of hypothesis-driven microarrays. With the advent of next-generation sequencing (NGS) in the last decade, researchers have harnessed the power of deep sequencing to further reveal the complexity of these small RNA species, which include many types of RNA such as microRNA (miRNA), short-interfering RNA (siRNA), and piwi-interacting RNA (piRNA), among others.
Ambion® RNA kits are a great way to isolate and purify your samples to help achieve high-quality capture of small RNA species. Leveraging the knowledge leadership of Ambion and integrating kits that isolate and purify small RNA species creates a complete solution that is optimized for small RNA analysis on the Ion PGM™ System. The scalability of the Ion PGM™ System allows you to include only a few or many samples per run, giving you the flexibility to quickly repeat experiments. Although other NGS platforms can also analyze small RNA, their ultrahigh-throughput format requires batching hundreds of samples per run, creating a less economical and less flexible solution when you only need to analyze a few samples, and delaying your time-to-results.
Ion Torrent™ next-generation sequencing systems offer better sensitivity, generating data superior to that obtained using microarrays at a comparable price. Choose the Ion PGM™ System for fast and accurate results for small RNA and targeted RNA studies. The Ion Proton™ System offers higher throughput, enabling you to scale from small RNA to whole-transcriptome analysis. Both systems surpass microarrays as a tool for expression analysis, with these advantages:
- Superior sensitivity, with a greater dynamic range, to detect more transcripts and subtle changes in gene expression
- Discrimination between transcripts from closely related genes or splice variants
- Detection of novel and fusion transcripts
- Digital output enabling results to be compared over time and between studies and labs
- Close correlation with TaqMan® Assay results
For Research Use Only. Not for use in diagnostic procedures.