Next-Generation Sequencing Validation
As every researcher knows, no single application or technique tells the whole story. Often, it is the thoughtful combination of approaches that delivers the insight needed to move research forward. We have interviewed three researchers who are successfully employing various capillary electrophoresis (CE) applications to fill in some of the gaps or ambiguities in their next-generation sequencing (NGS) data. Read "Case studies in complementary capillary electrophoresis and next-generation applications" to find more about the utility of combining NGS and CE data to gain a more complete picture.
Sanger sequencing provides fast and accurate analysis of the mutations present at a frequency of 20% or greater in a sample. The combination of the new BigDye® Direct Cycle Sequencing Kit and a 3500 Series Genetic Analyzer provide the most comprehensive and reliable sequencing data.
Sanger Sequencing for Genome Finishing
Sanger sequencing can perform in many situations where next-generation sequencing is limited, such as sequencing through difficult homopolymeric regions. Use the 3500 Genetic Analyzer for gap closure and to fill in regions of sequences that cannot be identified by next-generation sequencing approaches.
Primer Designer Tool
This fully automated online resource lets you quickly search for, configure and order primers for the Sanger confirmation step of your NGS workflow. The database consists of ~650,000 pre-designed primer pairs for re-sequencing the human exome and human mitochondrial genome.
Review our comprehensive list of publications describing Sanger sequencing and fragment analysis applications used in conjunction with next-generation sequencing.