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This recent paper1 published in Nature Protocols by the laboratory of Stephen Dalton at the University of Georgia describes how to reprogram patient fibroblasts to induced pluripotent stem cells (iPSCs) using the CytoTune®-iPS Reprogramming Kit, and then differentiate the iPSCs to neural crest stem cells in about 15 days using fully defined, feeder-free conditions.

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Products from Life Technologies featured in this article:

Reprogram

Sku Name Size Price Qty
A1378001 CytoTune®-iPS Sendai Reprogramming Kit 1 pack USD 1,998.00

Culture

Sku Name Size Price Qty
17104019 Collagenase, Type IV, powder 1 g USD 215.00
A1000701 StemPro® hESC SFM 1 kit USD 263.00
10565018 DMEM/F-12, GlutaMAX™ supplement 500 mL USD 38.10
21985023 2-Mercaptoethanol (55 mM) 50 mL USD 33.90
11107018 Transferrin, Bovine (Holo form), lyophilized 100 mg USD 113.00
PHG0023 FGF-Basic (AA 10-155) Recombinant Human Protein 1 mg USD 1,304.00
A1413202 Geltrex™ LDEV-Free, Reduced Growth Factor Basement Membrane Matrix 5 mL USD 181.00
11220035 Waymouth's Medium 500 mL USD 31.20
10828028 KnockOut™ Serum Replacement 500 mL USD 277.00
23181010 StemPro® EZPassage™ Disposable Stem Cell Passaging Tool 10 units USD 195.00

Characterize

Sku Name Size Price Qty
411000 TRA-1-60 Monoclonal Antibody, Mouse (cl.A) 100 µg USD 299.00
A21126 Alexa Fluor® 633 Goat Anti-Mouse IgG1 (γ1) 250 µL USD 159.00
A21042 Alexa Fluor® 488 Goat Anti-Mouse IgM (µ chain) 250 µL USD 159.00
V22886 Vybrant® DiO Cell-Labeling Solution 1 mL USD 112.00

Differentiate

Sku Name Size Price Qty
A1007201 StemPro® Osteogenesis Differentiation Kit 1 kit USD 135.00
A1007001 StemPro® Adipogenesis Differentiation Kit 1 kit USD 135.00
A1007101 StemPro® Chondrogenesis Differentiation Kit 1 kit USD 142.00


1Menendez L, Kulik MJ, Page AT et al. (2013) Directed differentiation of human pluripotent cells to neural crest stem cells. Nat Protoc Jan;8(1):203-12. Epub 2013 Jan 3.