StemPro Accutase Cell Reagent
StemPro® Accutase® is a marine-origin enzyme with proteolytic and collagenolytic activity for the detachment of primary and stem cell lines and tissues.

Gentle Dissociation Product for Sensitive Cells

  • Efficient - gently detach adherent cells within minutes
  • Convenient and cost effective - stable when frozen, refrigerated, and at room temperature
  • Maximum viability - standardized protocols for human embryonic stem cell (hESC) lines and human neural stem cell (hNSC) lines either in adherent or neurosphere suspension cultures

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Use With Embryonic and Neural Stem Cell Lines

StemPro® Accutase® Cell Dissociation Agent:

  • Shows high cell viability when tested with multiple embryonic stem cell and neural stem cell lines
  • It maintains normal morphology and equivalent gene expression markers of hESC and hNSC
  • Can dissociate suspension cultures such as neural stem cell spheroids, embryoid bodies, and floating sphere colonies
  • Dissociation of NSC on CELLstart™ in StemPro® NSC SFM shows a mean viability of 90% after dissociation with StemPro® Accutase® reagent

hESC’s Passaged with StemPro® Accutase®

StemPro® Accutase® allows single cell passaging of hESC when cultured in StemPro® hESC SFM (Figure 1).

Accutase cultures

Figure 1 - Robust single cell passaging of hESC’s.  BGO2 cells were disaggregated to single cells with StemPro® Accutase® reagent and plated in StemPro® hESC SFM on a Geltrex™ substrate.  Images taken 1.5 and 20 hours after plating indicate that cells migrate to form micro colonies.  These cultures expand to form monolayers of undifferentiated cells after 5-6 days of growth.

hNSC’s Passaged with StemPro® Accutase®

StemPro® Accutase® allows efficient dissociation of hNSC’s cultured in adherent and neurosphere suspension cultures (Figure 2).



Cells in Accutase® reagent

Cells in Accutase® reagent

Panel A

Panel B

Figure 2 - Dissociation of human neurosphere.  Human neurosphere (A) dissociated into single cell culture (B) using 1 ml of StemPro® Accutase® reagent for 10 minutes.

Ordering information

Sku Name Size Price Qty
A1110501 StemPro® Accutase® Cell Dissociation Reagent 100 mL USD 37.60
23181010 StemPro® EZPassage™ Disposable Stem Cell Passaging Tool 10 units USD 195.00
A1014201 CTS™ CELLstart™ Substrate 2 mL USD 236.00