• Fast
  • Requires less sample
  • Detect 4 early differentiation markers in one PCR reaction

Stem cell characterization made easy

Human Tri-lineage Multiplex PCR Kit:

  • Rapidly and reliably performs routine characterization of human embryonic stem cells (hESCs) and human embryonal carcinoma stem cells (hECs).
  • Detects markers for the undifferentiated state and all three embryonic lineages in one PCR reaction using a convenient multiplex RT-PCR assay.
  • Requires smaller sample volumes than traditional characterization methods.
  • Contains all the reagents necessary to quickly isolate RNA, make cDNA and perform PCR to detect the expression of differentiation markers.

Early Differentiation Markers

The STEMPRO® EZChek™ Kit contains primers to detect the following human genes in one multiplex PCR reaction:

  • Oct4/Pou5f1: marker for the pluripotent state of hESCs and hECs
  • AFP: endoderm lineage marker
  • ACTC1: mesoderm lineage marker
  • Sox1: ectoderm lineage marker
  • GAPDH: internal RNA standard for normalizing mRNA levels

Applications of the STEMPRO® EZChek™ Kit

This figure illustrates the characterization of BG01v human embryonic stem cells and a BG01v 21-day embryoid body (EB) control sample using the STEMPRO® EZChek™ Kit. BG01v human embryonic stem cells express only the pluripotency marker while in the BG01v EB control sample the expression of the pluripotency marker is down-regulated and the differentiation markers are induced.

Need a control for your characterization experiments?
Use the BG01V/hOG Cells Variant hESC hOct4-GFP Reporter Cells

Components of the STEMPRO® EZChek™ Kit

The Kit contains the following components:

  • PureLink™ Micro-to-Midi™ Total RNA Purification System: rapidly and reliably isolate high-quality total RNA from your hESCs or hECs.
  • SuperScript® III Reverse Transcriptase: provides high yields of first-strand cDNA in a convenient high-throughput supermix format.
  • STEMPRO® EZChek™ Human Tri-Lineage Primer Mix: contains primers for detecting five human genes in one multiplex PCR reaction.

Ordering Information

Sku Name Size Price Qty
23191050 StemPro® EZChek™ Human Tri-Lineage Multiplex PCR Kit 100 reactions USD 1,888.00
12344040 AccuPrime™ Pfx SuperMix 200 reactions USD 417.00
R7799105 BG01V/hOG Human Embryonic Stem Cells (hESCs) 2 x 106 cells USD 1,354.00
10816015 10X BlueJuice™ Gel Loading Buffer 3 x 1 mL USD 42.00
18068015 DNase I, Amplification Grade 100 units USD 118.00
10328011 RNase AWAY™ Decontamination Reagent 250 mL USD 58.00
18080400 SuperScript® III First-Strand Synthesis SuperMix 50 reactions USD 452.00
21985023 2-Mercaptoethanol (55 mM) 50 mL USD 33.90
17104019 Collagenase, Type IV, powder 1 g USD 215.00
14190144 DPBS, no calcium, no magnesium 500 mL USD 18.35
PHG0261 FGF-Basic (AA 1-155) Recombinant Human Protein 100 µg USD 592.00
A1000701 StemPro® hESC SFM 1 kit USD 263.00
15250061 Trypan Blue Solution, 0.4% 100 mL USD 14.90
12604013 TrypLE™ Express Enzyme (1X), no phenol red 100 mL USD 21.90