Photo of cardiac differentiation media bottles

We offer researchers an array of products designed to simplify cardiac differentiation. Our culture systems, reprogramming technologies, and characterization tools are the key components for the workflows needed to easily and efficiently generate beating cardiomyocytes.

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Generate contracting cardiomyocytes with these simple steps:

  1. Efficiently reprogram somatic cells to pluripotent stem cells (PSCs) with the CytoTune®-iPS 2.0 Reprogramming Kit.
  2. Culture PSCs feeder-free with Essential 8™ Medium.
  3. Generate beating cardiomyocytes in as few as 8 days with the PSC Cardiomyocyte Differentiation Kit.
  4. Effectively visualize cardiomyocyte markers with the tools you need in one box.

Chart showing workflow for cardiomyocyte differentiation

Reprogram somatic cells

The CytoTune®-iPS 2.0 Reprogramming Kit offers the highest efficiency for iPSC generation. Quickly identify pluripotent stem cells using Alkaline Phosphatase Live Stain.

Culture PSCs

Essential 8™ Medium contains only the necessary components needed for maintaining pluripotency of your hPSCs before neural induction. Validate pluripotency and cell health during PSC experiments using Molecular Probes® immunocytochemistry and live staining kits—the only kits offering superior imaging of pluripotent stem cells in one box.

Differentiation to cardiomyocytes

Differentiation of hPSCs to cardiomyocytes has never been easier:

  • Easy format—three 1x media products used in succession, with no frozen storage, thawing, or mixing of media required
  • Simple protocol—three-step protocol for differentiation (Fig 1)
  • Robust results—obtain physiologically relevant cardiomyocytes expressing key markers and contracting in culture (Fig 2)
  • Superior imaging—visualize cardiomyocyte markers with the tools you need in one box

Chart showing steps for using the PSC Cardiomyocyte Differentiation KitFigure 1. After expansion of pluripotent stem cells, cardiomyocytes are differentiated using the PSC Cardiomyocyte Differentiation Kit in three simple steps. Cells can be characterized after differentiation using the Human Cardiomyocyte Immunocytochemistry Kit.

Cell image showing cardiac marker staining  

Figure 2. iPSCs were differentiated for 14 days using the PSC Cardiomyocyte Differentiation Kit.
The cells were stained using the Human Cardiomyocyte Immunocytochemistry Kit for the following markers: NKX2.5 (red) for early cardiac mesoderm and TNNT2/cTNT (green) for cardiomyocytes along with DAPI nuclear DNA counterstaining (blue).

Watch Cardiomyocytes Video

Contracting cardiomyocytes using PSC Cardiomyocyte Differentiation Kit

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