RNA interference (RNAi) Gene Specific Silencing
Figure 1. RNAi Mechanism.
RNAi has been used as a tool by scientists to understand gene function in Caenorhabditis elegans and Drosophila. In these organisms, RNAi can be induced by introducing long dsRNA complementary to the target mRNA to be degraded. In mammalian cells and organisms, however, introducing dsRNA longer than 30 bp activates a potent antiviral response. To circumvent this, siRNAs are used to induce RNAi in mammalian cells and organisms.
In the last few years, siRNAs have been used in a number of different experimental settings to silence gene expression. In some, chemically synthesized or in vitro transcribed siRNAs have been transfected into cells, injected into mice, or introduced into plants (e.g. by a particle gun). In others, siRNAs have been expressed endogenously from siRNA expression vectors or PCR products in cells or in transgenic animals.
In addition to their role in gene silencing, siRNAs have been determined to play diverse biological functions in vivo -- roles that include antiviral defense, transposon silencing, gene regulation, centromeric silencing, and genomic rearrangements. This functional diversity has exemplified the importance of siRNAs within cells and has also stirred interest in their detection across species and tissues.
2. Dillin A (2003) The specifics of small interfering RNA specificity. Proc Natl Acad Sci USA 100(11): 6289 6291.
3. Tuschl T (2002) Expanding small RNA interference. Nature Biotechnol 20: 446 448.