Description: This short article discusses the differences between direct incorporation and post-synthesis reaction methods for fluorescently labeling nucleic acids for array analysis.
RNA Amplification Tips
Description: Two tips for improved RNA recovery following RNA amplification.
The Basics In Vitro Transcription
Description: The ability to synthesize RNA in the laboratory is critical to many techniques.
Tips for Optimizing Bacterial Array Analysis
Description: Advice on the isolation of RNA from bacteria for array analysis, including the use of Ambion's RNAlater™ storage solution, RiboPure™ Bacteria RNA Isolation Kit, MicrobEnrich™ and MicrobExpress™ Kits.
Tips for Successful RNA Amplification
Description: Our recommendations for performing consistent and reproducible RNA amplifications for microarray analysis.
Using Excess Labeled aRNA for Microarray Validation
Description: Amplified RNA is shown to be suitable for TaqMan® Gene Expression Assays. This means that the excess amplified RNA can be used to pre-screen microarray samples or to validate microarray results.
Using Non-Preferred Plate Shakers for MessageAmp™ Premier Reactions
Description: In this article we outline tips for improving recovery of MessageAmp™ Premier reaction products when using other (non-preferred) laboratory shakers.