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Monitor Phagocytosis In Real Time With the pH-sensitive pHrodo™ Red Dye

  • Specifically detect phagocytosis with a fluorogenic dye
  • Reduce signal variability and improve timing in sensitive experiments
  • Multiplex with green dyes such as GFP, fluo-4, and calcein


pHrodo™ Red is a novel rhodamine-based fluorogenic dye that dramatically increases in fluorescence as its surroundings become more acidic. The unique pHrodo™ system measures phagocytosis and endocytosis based on acidification of the particle or protein conjugates as they are ingested by cells.

Cytokines produced by Th1 cells stimulate the phagocytosis and destruction of microbial pathogens. Researchers can use the pHrodo™ Red succinimidyl ester or protein labeling kit to label their own microbial pathogen. Additionally, expertly prepared labeled bacteria are also available.

With a simple, no-cell background subtraction method, a large and specific signal is obtained from cells that have ingested the particles, providing a specific index of phagocytosis with a variety of pretreatments or conditions. This system offers key advantages over the classical nonfluorogenic indicators of bacterial uptake that require washing and quenching steps (J Immunol Methods 60:115 (1983); J Immunol Methods 162:1 (1993)).

Learn more at www.lifetechnologies.com/phrodo.

 

 Figure 1. Time-lapse images showing internalization and acidification of the pHrodo™ E.coli BioParticles® conjugate during phagocytosis by murine J774A.1 cells. Using excitation with a 561 nm laser, fluorescence images were recorded in the 570–699 nm emission range, and white-light DIC images were recorded on a separate PMT. Images were collected every 30 sec for 83.8 min (not all images are shown) using a Leica® TCS SP5 laser confocal microscope employing a 63x (NA 1.4) oil objective and the resonant galvanometer scanner mode (8,000 Hz). Image contributed by Lucy Deriy and Deborah Nelson, University of Chicago.

For Research Use Only. CAUTION: Not intended for human or animal therapeutic or diagnostic use.

Ordering Information

Sku Name Size Price Qty
P35363 pHrodo™ Red Microscale Labeling Kit 1 kit CNY 5,430.00
P36600 pHrodo™ Red, succinimidyl ester (pHrodo™ Red, SE) 1 mg CNY 6,360.00
P35361 pHrodo™ Red E. coli BioParticles® Conjugate for Phagocytosis 5 x 2 mg CNY 3,800.00
A10025 pHrodo™ Red E. coli BioParticles® Phagocytosis Kit for Flow Cytometry 1 kit CNY 13,290.00
A10010 pHrodo™ Red S. aureus Bioparticles® Conjugate for Phagocytosis 5 x 2 mg CNY 3,800.00

New IL-27 ABfinity™ Recombinant Antibody for Studying Th1 Differentiation

  • ABfinity™ recombinant antibodies are screened to be sensitive and specific
  • Enables consistent performance for your assays
  • Consistent product from lot to lot


ABfinity™ recombinant rabbit antibodies are produced from specific recombinant clones, so antibody performance is consistent over time. Lot-to-lot variability, commonly a problem with polyclonal products, is minimized with ABfinity™ antibodies. In addition to the monoclonal versions of our ABfinity™ antibodies, we offer oligoclonal versions of several of these specificities. Oligoclonal ABfinity™ products are a mixture of recombinant monoclonal antibodies and offer the improved signal strength you get from polyclonal antibodies combined with the highly reproducible results you get from monoclonals.

Interleukin 27 (IL-27) is a cytokine belonging to the IL-12 family. It is a heterodimeric cytokine comprising the EBI3 and p28 subunits. IL-27 is produced by activated dendritic cells and macrophages in response to Toll-like receptor ligands and proinflammatory cytokines. It promotes CD4+ T-cell differentiation to the Th1 lineage by inducing expression of the transcription factor T-bet and up-regulating IL-12Rβ2, which in turn suppresses Th2 and Th17 differentiation and proliferation.

 

 Figure 2. Western blot analysis was performed on active recombinant human IL-27 protein. A band corresponding to human IL-27 at ~27 kDa was detected using IL-27 ABfinity™ Recombinant Rabbit Monoclonal Antibody (Cat. No. 701073) at a concentration of 1 μg/mL.


ABfinity™ Stem Cell Antibody Selection Guide

Description Reactivity Monoclonal Cat. No. Oligoclonal Cat. No.
IL-27 ABfinity™ Recombinant Rabbit Antibody, purified, 100 µgHuman 701073 710175

For Research Use Only. CAUTION: Not intended for human or animal therapeutic or diagnostic use.

Gibco® Recombinant IL-12

  • High biological activity
  • High purity
  • Proven compatibility with Gibco® media

 
Interleukin 12 (IL-12) is a heterodimeric cytokine composed of a disulfide-bound 35 kDa subunit (p35) and a 40 kDa subunit (p40). IL-12 is produced primarily by monocytes, macrophages, dendritic cells, and B cells. Its major functions include induction of IFN-γ production from natural killer (NK) cells and T cells, enhancement of cytotoxicity of NK and cytotoxic T cells, and differentiation of naive T cells into Th1 effectors. Life Technologies provides Gibco® recombinant human IL-12 as well as many other cytokines.

Gibco® cytokines and growth factors are high-purity recombinant proteins with high bioactivity. Gibco® growth factors are of the highest quality because each protein is analyzed for purity along with structural homogeneity to help ensure that a biologically active protein is produced. And Gibco® cytokines and growth factors have been bioassayed with Gibco® media. Get your cytokines and growth factors from the media company you use and trust.

 

Description Cat No. (1 mg) Cat No. (10mg)
IL-12 ABfinity™ Recombinant Rabbit Antibody, purified, 100 µg PHC1123 PHC1124

For Research Use Only. CAUTION: Not intended for human or animal therapeutic or diagnostic use.

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