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Measure Human β-amyloid Proteins for Alzheimer’s Disease Research

  • Simple—easy-to-run sandwich ELISA
  • Rapid—4-hour incubation protocol and same-day results
  • Convenient—precoated, removable 8-well strips


Alzheimer’s disease (AD) is characterized by the presence of intracellular neurofibrillary tangles and extracellular plaques in the brain, and the major component of these plaques is beta-amyloid peptide (Aβ). Aβ peptide can range from 39 to 43 amino acids in length and is formed after sequential cleavage of the amyloid precursor protein (APP). The predominant forms are Aβ40 and Aβ42, with Aβ40 being the more common of the two and Aβ42 being more associated with disease states.

We offer sandwich ELISA kits to quickly detect and quantify human Aβ in cell culture supernatants, cerebrospinal fluid (CSF), and tissue homogenates for various research models of Alzheimer’s disease. ELISA kits allow results to be collected easily and reproducibly, and calibrated standard curves are provided to accurately quantify the level of protein in each experimental run.

 

  

 Figure 1. With a few incubation steps and washes, you can typically have quantitative results in 4 hours.

 

CSF Dilution
Aβ42
Measured
(pg/mL)
Expected
(pg/mL)
%
Expected
1/468.99
1/836.9034.50107%
1/1619.4317.25113%
1/329.098.62105%

Table 1. Measuring Aβ42 in dilutions of cerebrospinal fluid (CSF) using the Aβ42 High Sensitivity ELISA Kit. Human CSF containing Aβ42 at a measured concentration of 280 pg/mL was initially diluted 1:1, then serially diluted in Standard Diluent Buffer over the range of the assay.

For Research Use Only. CAUTION: Not intended for human or animal therapeutic or diagnostic use.

Ordering Information

Sku Name Size Price Qty
KHB3481 Aβ40 Human ELISA Kit 96 assays USD 634.00
KHB3441 Aβ42 Human ELISA Kit 96 assays USD 634.00
KHB3544 Aβ 42 Human Ultrasensitive ELISA Kit 96 assays USD 680.00

New ABfinity™ Recombinant Antibodies for Analyzing Neuronal Cells

  • ABfinity™ stem cell recombinant antibodies are screened to be sensitive and specific
  • Consistent performance for your assays
  • Consistent product from lot to lot


ABfinity™ recombinant rabbit antibodies are produced from specific recombinant clones, so antibody performance is consistent over time. Lot-to-lot variability, commonly a problem with polyclonal products, is minimized with ABfinity™ antibodies.

Over the past year we have released several new ABfinity™ recombinant antibodies to support neurobiology research. These antibodies are against a range of markers, including stem cell–related proteins like nucleostemin and musashi-1. In addition to the monoclonal versions, we offer oligoclonal versions of several of these specificities. Oligoclonal ABfinity™ products are a mixture of recombinant monoclonal antibodies and offer the improved signal strength you get with polyclonal antibodies combined with the highly reproducible results you get from monoclonals.

 

 Figure 2. Immunocytochemistry analysis of HeLa cells stained with (a) Nucleostemin ABfinity™ Recombinant Rabbit Oligoclonal Antibody followed by Alexa Fluor® 488 goat anti–rabbit IgG as secondary, (b) DAPI nuclear stain, (c) Alexa Fluor® 594 phalloidin actin stain. Panel d shows the composite image.


ABfinity™ Stem Cell Antibody Selection Guide

Description Validation* Monoclonal Cat. No. Oligoclonal Cat. No.
Alpha-SynucleinAssay/WB/ICC 701085 710110
Beta Amyloid (1-40)Assay/IHC -- 710174
Beta Amyloid (1-42)Assay/ICC 700254 --
TauAssay/WB -- 710080
Tau (pS199)Assay/WB 701054 710124
Tau (pT231)Assay/WB 701056 710126
GSK3b (pS9)Assay/WB 701069 710100
NucleosteminAssay/WB/ICC 701157 710188
PitX3Assay/WB/ICC 701181 710212
Musashi-1Assay/WB/ICC -- 710237

*WB = Western blot, ICC = Immunocytochemistry

For Research Use Only. CAUTION: Not intended for human or animal therapeutic or diagnostic use.

Fluorescent Probes for Neural Tracing and Anatomy

  • Extensive selection of probes for neuronal tracing, anatomy, and function
  • Conjugates of bright and photostable Alexa Fluor® dyes help you get the most information from your samples
  • Proven utility in hundreds of publications

 
We offer a comprehensive set of Molecular Probes® neural tracing stains. Whether you’re defining neuronal cell connectivity, monitoring translocation through gap junctions, following cells through generational divisions, or identifying specific cells of interest, we have fluorescent and biotinylated probes to help you get the data you’re looking for. All of our Molecular Probes® fluorescent dyes and stains meet stringent quality specifications, and data obtained with them have been featured in hundreds of publications over more than three decades. 

 

 Figure 3. Coronal section of mouse hippocampus. A 14 µm coronal section of mouse hippocampus stained with an anti–α-tubulin antibody labeled with the Zenon® Alexa Fluor® 488 Mouse IgG1 Labeling Kit. The section was counterstained with the NeuroTrace® 530/615 Red Fluorescent Nissl Stain to visualize neuronal cell bodies, and Hoechst 33258 (Cat. No. H1398, H3569, H21491) to stain nuclei.

For Research Use Only. CAUTION: Not intended for human or animal therapeutic or diagnostic use.

Ordering Information

Sku Name Size Price Qty
C7025 CellTracker™ Green CMFDA (5-Chloromethylfluorescein Diacetate) 20 x 50 µg USD 306.00
C2925 CellTracker™ Green CMFDA (5-Chloromethylfluorescein Diacetate) 1 mg USD 256.00
C34552 CellTracker™ Red CMTPX 20 x 50 µg USD 308.00
D22910 Dextran, Alexa Fluor® 488; 10,000 MW, Anionic, Fixable 5 mg USD 285.00
D1956 Dextran, Biotin, 10,000 MW, Lysine Fixable (BDA-10,000) 25 mg USD 210.00
C22841 Cholera Toxin Subunit B (Recombinant), Alexa Fluor® 488 Conjugate 500 µg USD 548.00
C34775 Cholera Toxin Subunit B (Recombinant), Alexa Fluor® 488 Conjugate 100 µg USD 188.00
C22842 Cholera Toxin Subunit B (Recombinant), Alexa Fluor® 594 Conjugate 500 µg USD 548.00
L453 Lucifer Yellow CH, Lithium Salt, 25 mg 25 mg USD 97.00
N21480 NeuroTrace® 500⁄525 Green Fluorescent Nissl Stain - Solution in DMSO 1 mL USD 161.00
N21482 NeuroTrace® 530⁄615 Red Fluorescent Nissl Stain - Solution in DMSO 1 mL USD 161.00
N21479 NeuroTrace® 435/455 Blue Fluorescent Nissl Stain 1 mL USD 156.00
Q21021MP Qtracker® 655 Vascular Labels 200 µL USD 481.00
Q25021MP Qtracker® 655 Cell Labeling Kit 1 kit USD 628.00
Q25041MP Qtracker® 525 Cell Labeling Kit 1 kit USD 628.00

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