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Easily Measure Focal Adhesion Kinase (FAK) With Our phosphoELISA™ Kits

  • Sensitive assays—allow for detection of low protein levels
  • Fast and easy—4-hour incubation protocol
  • Improved data—allow generation of quantitative data



FAK is under investigation in several areas of research, including studies of cell adhesion and migration, growth factor–mediated migration, differentiation, cell cycle, apoptosis, and cancer. FAK is overexpressed in breast, colon, and thyroid cancers, possibly contributing to some features of the malignant phenotype (i.e., increased migration and invasion of surrounding stroma). 
Integrins play an important role in cell attachment to other cells or to the extracellular matrix as well as in signal transduction. Focal adhesion kinase (FAK) is a nonreceptor protein tyrosine kinase that localizes to focal adhesions. In response to integrin engagement, FAK is autophosphorylated at tyrosine residue 397. This autophosphorylation event creates high-affinity binding sites for the SH2 domains of several important signaling proteins. 

Our phosphoELISA™ kits offer the ability to quickly measure signaling proteins in cell lysates using a convenient 96-well format. Just process your samples as you would for a western blot, then add them to an ELISA plate, run the assay, and get quantitative results in as little as 4 hours.

  • Learn More About phosphoELISA™ Kits
Specificity of the FAK phosphoELISA™ assay
The specificity of the FAK phosphoELISA™ assay for phosphorylated FAK [pY397] was confirmed by peptide competition.
Only the phosphopeptide containing the phosphorylated tyrosine 397 could block the ELISA signal. The nonphosphorylated peptide sequence or other phosphopeptides from the FAK sequence did not block the signal.
For research use only. Not intended for any animal or human therapeutic or diagnostic use.
Ordering Information

Product
Quantity
Cat. No.
FAK [pY397] ELISA KIT
96 tests
KHO0441
FAK (TOTAL) ELISA KIT
96 tests
KHO0431

How Does p53 Influence Tumor Progression With Integrin Signaling?

  • ABfinity™ p53 [pS15] rabbit recombinant monoclonal antibody for sensitive detection
  • Designed for consistent performance for your assays
  • Consistent product from lot to lot



ABfinity™ recombinant rabbit monoclonal antibodies are produced from specific recombinant clones, so antibody performance is consistent over time. ABfinity™ antibodies are available for many targets, including p53 (gene ID 23491729).
p53 can drive tumor progression through integrin recycling. p53 can disrupt direction of migration and other metastatic characteristics through integrin and epidermal growth factor (EGFR) signaling. ABfinity™ recombinant monoclonal antibodies are ideal reagents for signaling pathway research. ABfinity™ technology helps to ensure consistent antibody performance, so you don’t have to reoptimize your assay with each new lot.

  • Learn More About ABfinity™ Recombinant Rabbit Monoclonal Antibodies
Rabbit anti-p53 [pS15]
Rabbit anti-p53 [pS15] (1 µg/mL) was used to label p53 [pS15]
in U2OS cells with no pretreatment (left) or pretreated with 0.25 µg/mL camptothecin (right). Alexa Fluor® 488 goat anti-rabbit at 1:1,000 was used as secondary antibody.
For research use only. Not intended for any animal or human therapeutic or diagnostic use.

Gibco® Recombinant Proteins for Integrin Pathway Research

  • High biological activity
  • High purity
  • Proven compatibility with Gibco® media



Gibco® recombinant proteins are useful for a wide variety of pathway research investigations. Gibco® recombinant proteins have been bioassayed in cells cultured in Gibco® media. So if you’re already using Gibco® media, you can have more confidence in the compatibility of these reagents in your cell culture–based assays.

Integrins are important in transmitting signals from the extracellular matrix (ECM) to cells. The ECM is involved in movement of cells during wound healing and embryonic development, and also relays important information to the cells. Two key ligands found in the ECM are fibronectin and vitronectin. These glycoproteins bind to integrins at the surface of the cell and play a role in a variety of biological processes.

Fibronectin plays a role in cell adhesion, growth, migration, and differentiation and is involved in diverse processes impacting wound healing, development, cancer, and fibrosis.
Vitronectin plays a role in cell adhesion and binds to serpin serine protease inhibitors. It may be involved in cancer and hemostasis.

Gibco® cytokines, growth factors, and proteins are high-purity recombinant proteins with high bioactivity. To help ensure Gibco® proteins are of the highest quality, each protein is analyzed for purity along with structural homogeneity so that a biologically active protein is produced.

For research use only. Not intended for any animal or human therapeutic or diagnostic use.
Ordering Information

Product
Qty.
Cat. No.
Vitronectin Natural Human
100 µg   
PHE0011
Fibronectin Natural Human
1 mg
PHE0023

Investigate cAMP Influence on Integrin-Mediated T Cell Adhesion

  • Broad dynamic range—0.06 to 6,000 pmol (4–5 logs)
  • Flexible—can be used in manual or automated, high-throughput formats
  • Simple—sample dilution not necessary



Our competitive, heterogeneous cAMP-Screen® and cAMP-Screen Direct® immunoassays measure cAMP directly from cell lysates at levels as low as 60 fmol to 6 µmol, over 4 to 5 logs, without requiring any sample dilution.

Detection is complete typically within 90 minutes, utilizes chemiluminescence, and is amenable to use in automated, high-throughput screening applications.

Cyclic adenosine monophosphate (cAMP) is an important second messenger in many signal transduction pathways linking activation of cell surface membrane receptors to intracellular responses and, ultimately, to changes in gene expression.
Several proteins, including Epac proteins (exchange protein directly activated by cAMP) and Rap1, involved with integrin-mediated T cell adhesion, are directly modulated by cAMP.

The sensitivity and simplicity of the cAMP-Screen Direct® assay enables researchers to measure small changes in cAMP levels as they investigate integrin-mediated T cell adhesion.

For research use only. Not intended for any animal or human therapeutic or diagnostic use.

Alexa Fluor® Dye Secondary Antibodies—An Imaging Tradition

  • Over 10 years of published results
  • Brighter
  • More photostable



Through superior performance, Alexa Fluor® dyes have been contributing to illuminating research for over 10 years. And to help with experiment planning, Alexa Fluor® dyes are supported by an experienced technical support team, >30,000 published references, application and experimental tips, and protocols.

Integrins are cell surface receptors that interact with the extracellular matrix and mediate intracellular signals for cell shape, mobility, and

progression through the cell cycle. After incubating the sample with the anti-integrin primary antibody of your choice, you can detect it with any of a number of fluorescent Alexa Fluor® dye–labeled secondary antibodies.

Because of their superior brightness and photostability, Alexa Fluor® conjugates are superior to most conventional fluorescent secondary reagents and are the detection reagents of choice for many fluorescence-based immunoassays.

Two-color confocal image of a human epidermal whole mount
Two-color confocal image of a human epidermal whole mount.
ß1 integrin was visualized with the monoclonal antibody P5D2 labeled with the green-fluorescent Alexa Fluor® 488 dye using the Alexa Fluor® 488 Monoclonal Antibody Labeling Kit. a6 integrin was labeled using a mouse monoclonal antibody visualized with Alexa Fluor® 594 goat anti–mouse IgG antibody and pseudocolored blue. Image contributed by Uffe Birk Jensen, University of Aarhus, Denmark.
For research use only. Not intended for any animal or human therapeutic or diagnostic use.
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