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New Antibodies to Measure AS160 [pT642] in the Study of Metabolic Risk Factors for Insulin Sensitivity and Diabetes

  • ABfinity™ AS160 [pT642] rabbit recombinant monoclonal and oligoclonal antibodies for sensitive detection
  • Designed for consistent performance for your assays
  • Consistent product from lot to lot


ABfinity™ recombinant rabbit monoclonal antibodies are produced from specific recombinant clones, so antibody performance is consistent over time. ABfinity™ antibodies are available against many targets, including AS160 and AMPK alpha.

The AKT substrate AS160 (gene ID 9882) is implicated in signaling cascades that contribute to insulin resistance (especially in individuals with elevated levels of inflammatory markers and increased metabolic risk factors) by inhibiting insulin signaling protein

phosphorylation. When AS160 is phosphorylated at threonine 642 in the presence of higher HDL levels, insulin resistance is reduced. Glucose intake and exercise affect phosphorylation of AS160 T642 and AMPK alpha. T172, and these rapidly changing levels of phosphorylation contribute to many downstream signaling pathways, implicating these critical phosphorylation sites in the understanding of insulin resistance and type 2 diabetes. ABfinity™ recombinant monoclonal antibodies are ideal reagents for signaling pathway research. The ABfinity™ technology helps to ensure consistent antibody performance so you don’t have to reoptimize your assay with each new lot.

AS160 [pT642] ABfinity™ Recombinant Rabbit Monoclonal Antibody

Figure 1.
AS160 [pT642] ABfinity™ Recombinant Rabbit Monoclonal Antibody recognizes a ~160 kDa protein in western blot analysis of serum-starved HeLa cells (whole cell extract, 80 µg per lane).
For research use only. Not intended for any animal or human therapeutic or diagnostic use.


Alexa Fluor® Secondary Antibodies—An Imaging Tradition

  • Over 10 years of published results
  • Choice of human, mouse, rabbit, rat, chicken, goat, guinea pig, hamster, and sheep reactivities
  • Highly cross-adsorbed lots available to minimize cross-reactivity


Alexa Fluor® dyes have been illuminating research for over 10 years. In addition to superior performance, these dyes are backed by an experienced, problem-solving technical support team, >30,000 published references, application and experimental tips, and protocols to help with experimental planning.

Examination of the expression patterns of proteins can provide important support to traditional biochemical data. Alexa Fluor® dyes are conjugated to a selection of secondary antibodies and other detection reagents to enable you to observe the distribution of your target protein within cells or in various tissues. Alexa Fluor® dyes provide a time-tested solution to your imaging needs.

Possessing good brightness and photostability properties, Alexa Fluor® conjugates are superior to most conventional fluorescent secondary reagents and are the detection reagents of choice for use with many fluorescence-based immunoassays.

Mouse intestine cryosection showing basement membranes

Figure 2. Mouse intestine cryosection showing basement membranes labeled with chicken IgY anti-fibronectin primary antibody and Alexa Fluor® 647 goat anti–chicken IgG secondary antibody (pseudocolored purple).
Goblet cells and crypt cells were labeled with Alexa Fluor® 594 wheat germ agglutinin (red). The microvillar brush border and smooth muscle layers were visualized with Alexa Fluor® 488 phalloidin (green). Nuclei were counterstained with SYTOX® Blue Nucleic Acid Stain (blue).
For research use only. Not intended for any animal or human therapeutic or diagnostic use.


Measuring Biomarkers in Metabolism Research

  • Accurately measure hormones circulating in blood
  • Gold-standard, trusted ELISA method
  • Rapid, easy-to-perform assays

Insulin, leptin, and adiponectin are polypeptide hormones involved in metabolic processes. Adipocytes express a variety of adipocytokines that function in the homeostatic control of glucose and lipid metabolism. Insulin, released into the blood by the beta cells of the islets of Langerhans in the pancreas, regulates secretion of many of these adipocytokines in response to changes in energy balance.

Typically after eating, insulin is released in response to rising levels of blood glucose.

 

High glucose levels (occurring either because the body does not produce enough insulin or because cells do not respond to insulin) define diabetes. Adiponectin is exclusively secreted from adipose tissue into the blood and is very abundant in plasma relative to other hormones. Levels of adiponectin are lower in diabetics than in nondiabetics, and weight reduction significantly increases circulating levels. Leptin has an effect similar to that of adiponectin, but the two hormones perform complementary actions and can have additive effects.

By providing accurate and reliable methods to measure hormone levels in research samples, these assay kits are useful research tools that may allow researchers to better understand diseases.

  • Learn More About Invitrogen™ ELISA Kits
Assay procedure for Hu Insulin ELISA Kit

Figure 3. Assay procedure for Hu Insulin ELISA Kit.
Note that incubation time is only 45 minutes.
Ordering Information

Product
Species
Qty.
Cat. No.
Hu Insulin ELISA Kit
Human
96 tests
KAQ1251
Hu Leptin ELISA Kit
Human
96 tests
KAC2281
Ms Leptin ELISA Kit
Mouse
96 tests
KMC2281
Hu Adiponectin ELISA KitHuman
96 tests
KHP0041
Ms Adiponectin ELISA KitMouse
96 tests
KMP0041
Rt Adiponectin ELISA KitRat
96 tests
KRP0041

For research use only. Not intended for any animal or human therapeutic or diagnostic use.


Luminex® MAGPIX® System—Effective Alternative to ELISA for Insulin Receptors (IR)

  • Ideal immunoassay tool—simultaneous analysis of up to 50 proteins in limited volume of precious sample
  • Excellent performance—accurate, reproducible, and sensitive quantification of proteins
  • Reduces time and cost—better approach than multiple, single-analyte ELISAs or western blots
  • Easy to use—designed to work with broad and expanding range of Invitrogen™ assay kits


Enzyme-linked immunosorbent assays (ELISAs) have become the standard method for the quantitation of selected protein targets in biological samples. However, ELISAs have been found to be less than ideal for analyzing multiple proteins in single samples.

 

The MAGPIX® System is built on the well-known Luminex® xMAP® technology, one of the most popular and effective multiplexing technologies for simultaneous measurement of multiple biomarkers. This instrument provides an inexpensive benchtop immunoassay system for performing up to 50 assays on a single biological sample. The MAGPIX® System offers fast, accurate, and easily reproducible results, to help save time and money and preserve valuable samples. This versatile multiplexing platform can be used across a broad range of sample types and protein targets, making it applicable to multiple research settings.

Standard curve for quantitation of human TNF-alpha

Figure 4. The MAGPIX® System (A).
Standard curve for quantitation of human TNF-alpha in a tissue culture supernatant sample, measured with ELISA and an xMAP® assay. In this singleplex format, the xMAP® assay had excellent correlation with the ELISA (R² = 0.9443) (B).
For Research Use Only.  Not intended for any animal or human therapeutic or diagnostic use. © 2011 Life Technologies Corporation. All rights reserved.  The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners.