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Gibco® Recombinant Proteins for Studying TNF Pathways

  • High biological activity
  • High purity
  • Proven compatibility with Gibco® media


Gibco® recombinant proteins are highly purified bioactive proteins. Gibco® cytokines and receptors have been bioassayed with Gibco® media. Get your recombinant proteins from the media company you use and trust.

TNF-α, TNF-β/lymphotoxin, and TRAIL are proinflammatory cytokines that activate various signaling pathways, including the NF-κB signaling pathway. Upon TNF-α stimulation, IκBα is phosphorylated, which marks the protein for degradation, making NF-κB free to translocate to the nucleus to induce gene expression.

We offer bioactive recombinant TNF-α from human, mouse, rat, and swine for use in cell culture applications. We also offer recombinant human TNF-β/lymphotoxin, TRAIL, and soluble forms of TNF-RI and TNF-RII.

Gibco® cytokines and growth factors are high-purity recombinant proteins with high bioactivity. To help ensure Gibco® recombinant proteins are of the highest quality, each protein is analyzed for purity and structural homogeneity.

Jurkat cells treated with recombinant human TNF-α
Figure 1. Jurkat cells treated with recombinant human TNF-α.
The level of phosphorylated IκBα increased upon TNF-α stimulation. As expected, the total level of IκBα decreased upon stimulation due to proteasomal degradation. IκBα [pS32] and IκBα (total) levels were measured using the IκBα [pS32] Human ELISA Kit and IκBα (Total) Human ELISA Kit, respectively.
For research use only. Not intended for any animal or human therapeutic or diagnostic use.
Recombinant Interferon Selection Guide

TNF and TRAIL Products
Size
 Cat. No.
TNF-α Recombinant Human
10 μg
PHC3015
TNF-α Recombinant Human (Liquid)
10 μg
PHC3015L

More Products Listed Below

TNF and TRAIL Products (cont'd)
Size
 Cat. No.
TNF-α Recombinant Human
50 µg
PHC3016
TNF-α Recombinant Human
100 mg
PHC3011
TNF-α Recombinant Human
11 mg
PHC3013
TNF-α Recombinant Mouse
10 µg
PMC3014
TNF-α Recombinant Rat
10 mg
PRC3014
TNF-α Recombinant Swine
10 µg
PSC3014
TNF-β/Lymphotoxin Recombinant Human
10 µg
PHC3024
TRAIL Recombinant Human
50 mg
PHC1634

TNF Receptor Products
Size
 Cat. No.
Soluble TNF-RI Recombinant Human
20 µg
PHR3015
Soluble TNF-RII Recombinant Human
20 mg
PHR3025

Is TRAF3 Upregulated in Your Diseased Samples?

  • ABfinity™ TRAF3 rabbit recombinant monoclonal for sensitive detection
  • Consistent performance for your assays
  • Consistent product from lot to lot


ABfinity™ recombinant rabbit monoclonal antibodies are produced from specific recombinant clones so antibody performance is consistent over time. ABfinity™ antibodies are available for many targets, including TRAF3.

TNF receptor-associated factor 3 (TRAF3) is important in regulating immune responses in numerous pathways downstream of TNF signaling cascades, such as JNK and NFkB in T cell–mediated immunity.

ABfinity™ recombinant monoclonal antibodies are ideal reagents for signaling pathway reagents. ABfinity™ technology helps ensure consistent antibody performance, so you don’t have to reoptimize your assay with each new lot.

TRAF3 detected in HeLa cell lysates
Figure 2. TRAF3 detected in HeLa cell lysates.
For research use only. Not intended for any animal or human therapeutic or diagnostic use.
ABfinity™ Recombinant Rabbit Monoclonal Antibody Selection Guide

Product
Species
Quantity
Cat. No.
TRAF3 ABfinity™ Recombinant Rabbit Monoclonal Antibody - purified
Human
100 μg
700121
TNF-alpha mouse monoclonal antibody – biotin conjugated
Human
100 μg
AHC3419
TNF-alpha mouse monoclonal antibody
Human
500 μg
AHC3712
TNF-alpha mouse monoclonal antibody
Human
500 μg
AHC3912
TNF-RI mouse monoclonal antibodyHuman100 μgAHR3819
TNF-RI mouse monoclonal antibodyHuman500 μgAHR3912

Get Reliable Results and Save Time—Invitrogen™ Multiplex Bead-Based Luminex® Assays

  • Superior performance—enables accurate quantitation of TNF signaling pathway proteins
  • High quality—fully characterized and validated antibodies that deliver high specificity and sensitivity
  • Fast and easy—NEW magnetic bead–based Luminex® assays


We have developed analysis platforms and assay kits to measure a wide range of cytokine and signal transduction proteins. The Invitrogen™ Luminex® 200™ and FlexMAP 3D® systems are compact benchtop analyzers capable of performing up to 100 assays simultaneously in a single well of a 96-well microtiter plate (Luminex® 200™ system)

 

or up to 500 assays simultaneously in a single well of either a 96-well or 384-well microtiter plate (FlexMAP 3D® system).

Invitrogen™ Luminex® singleplex and multiplex assays provide a rapid and cost-effective alternative approach to the analysis of numerous key pathway proteins. These assays can be applied to a broad range of species (human, mouse, rat, and monkey) and sample types (serum, plasma, and tissue culture media). All lots of Invitrogen™ Luminex® singleplex and multiplex assays have been qualified using the Invitrogen™ Luminex® 200™ System and the FlexMAP 3D® System, which helps ensure superior analysis performance and reproducible results.

For research use only. Not intended for any animal or human therapeutic or diagnostic use.

Ordering Information

Product Cat. No.
Luminex® 200™ SystemMAP0200 Get a Quote
FlexMAP 3D® SystemFMD0000 Get a Quote
Luminex® Singleplex and Multiplex AssaysSee All Luminex Assays


Measure TNFα Using the Gold-Standard ELISA Method

  • Reliable measurements of TNFα in serum, plasma, or supernatant
  • Fast assay—as little as 2.5 hours of incubation time
  • Consistent results


Tumor Necrosis Factor Alpha (TNF-α), also called cachectin, is a cytokine mainly produced by monocytes and activated macrophages. TNF-α is defined by its participation in inflammatory activities, cell toxicity, and mediation of TNF-α tumor toxicity.

 

Accurate and precise measurements of TNF-α are critical in many research areas. Invitrogen™ ELISA kits provide an easy and reliable way to quantify the amount of TNF-α in your serum, plasma, or supernatant sample. Precoated ELISA kits are available with various species reactivities, including human, mouse, rat, swine, and monkey. Economy pack sizes are also available for your convenience. For more savings and flexibility to develop your own immunoassays, matched antibody pairs are also available.

Example of assay procedure for TNFα Mouse ELISA Kit
Figure 4. Example of assay procedure for TNFα Mouse ELISA Kit. Incubation time for this assay is only 2.5 hours.
For research use only. Not intended for any animal or human therapeutic or diagnostic use.
Ordering Information

ELISA Kits
Range
Sample Vol. Needed
Incubation Time
Cat. Info
TNFα Human ELISA Kit
15.6–1000 pg/mL
100 µL
4 hr
See All Kit Sizes
TNFα Ultrasensitive Human ELISA Kit0.5–32 pg/mL
50 µL
3 hr
See All Kit Sizes
TNFα Mouse ELISA Kit19.5–1,250 pg/mL
50 µL
2.5 hr
See All Kit Sizes
TNFα Rat ELISA Kit15.6–1000 pg/mL
50 µL
2.75 hr
See All Kit Sizes
TNFα Monkey ELISA Kit15.6–1000 pg/mL
50 µL
4 hr
See All Kit Sizes
TNFα Swine ELISA Kit23–1500 pg/mL

25 µL
4 hr
See All Kit Sizes

Antibody Pairs (User Flexibility)
Size
Cat. no.
TNFα Human Antibody Pair
10 plates
CHC1753
TNFα Mouse Antibody Pair10 platesCMC3013
TNFα Rat Antibody Pair10 platesCRC3013
TNFα Swine Antibody Pair10 platesCSC1753
© 2011 Life Technologies Corporation. All rights reserved. The trademarks mentioned herein are the property of Life Technologies Corporation or their respective owners.  Luminex 200 and FlexMap 3D are trademarks of Luminex Corporation.