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Pathway Focus: The Met Signaling Pathway
- ABfinity™ c-Met and EGFR rabbit recombinant monoclonal antibodies for sensitive detection
- Enable consistent performance for your assays
- Consistent product from lot to lot
ABfinity™ recombinant rabbit monoclonal antibodies are produced from specific recombinant clones so antibody performance is consistent over time. ABfinity™ antibodies are available for many targets, including c-Met and EGFR.
The c-Met receptor is a potential therapeutic target in some cancer models.
- Learn More About ABfinity™ Recombinant Rabbit Monoclonal Antibodies
Find Antibodies Using the Primary Antibody Search Tool
|Figure 1. Immunocytochemistry of A549 cells labeled with rabbit anti–c-Met.
|c-Met ABfinity™ Recombinant Rabbit Monoclonal Antibody – Purified
|c-Met [pY1230] ABfinity™ Recombinant Rabbit Monoclonal Antibody – Purified
|EGFR ABfinity™ Recombinant Rabbit Monoclonal Antibody – Purified||Human
|EGFR ABfinity™ Recombinant Rabbit Monoclonal Antibody – Purified||Rat, mouse, human||100 µg
|Alexa Fluor® 488 goat anti-rabbit (2 mg/mL)||rabbit
- Fast and easy assay protocols—perform and analyze your data in less than one day
- Superior performance—accurate, reproducible, and sensitive quantification of signaling proteins
- High quality—antibody-based assays with excellent specificity and sensitivity
To facilitate the analysis of STAT3 as well as a broad range of important signaling molecules, Life Technologies has developed a series of singleplex and multiplex immunoassays for the Luminex® platform. Samples containing targeted analytes bind to antibodies immobilized on color-coded polystyrene or magnetic beads.
The beads are then analyzed with the Luminex® 100™ or 200™ system or the FLEXMAP 3D® system. By monitoring the spectral properties of the beads and the amount of associated RPE fluorescence (from the secondary antibody conjugate),
A member of the tyrosine kinase superfamily, c-Met is the receptor for hepatocyte growth factor, also known as scatter factor (HGF/SF). Phosphorylation of tyrosines 1234 and 1235 of c-Met is required for activation of the receptor’s tyrosine kinase activity. Several signaling proteins are phosphorylated and/or localized in response to c-Met phosphorylation. STAT3 (signal transducer and activator of transcription 3) is phosphorylated on tyrosine residue 705. STAT3 mediates the expression of a variety of genes in response to cell stimuli, and thus plays a key role in many cellular processes such as cell growth and apoptosis.
- Learn More About Luminex® Assays
|Figure 2. HeLa cells were stimulated with 100 ng/mL IFNα for 20 minutes.
Untreated cells were used as a control. The results indicate that phosphorylation of STAT3 at tyrosine 705 increased following IFNα treatment.
|STAT3 [pY705] Singleplex Bead Kit
|Luminex® 100™ or 200™ System and FLEXMAP 3D® System
- Reliable—get quantitative results with validated kits
- Convenient—ready-to-use kits that come with standards and strip-well plates
- Fast—assay protocol in 4 hours
c-Met ELISA kits allow easy and quantifiable measurement of soluble c-Met or phosphorylated c-Met. c-Met is a receptor tyrosine kinase for hepatocyte growth factor (HGF). Soluble c-Met is a truncated form of the c-Met membrane receptor and can be measured in serum, plasma, or supernatant. Phosphorylated and total c-Met can be measured in cell lysate samples.
Receptor tyrosine kinases are important therapeutic inhibition targets for cancer research.
The c-Met ELISA kits are valuable assay tools for studying the effects of drug inhibitors on c-Met signaling. For normalizing c-Met content of the samples, the c-Met (Total) ELISA Kit is also available, which assays for c-Met independent of phosphorylation status. The assay is completed using a 4-hour incubation protocol.
- Learn More About ELISA Kits
|Figure 3. Treatment with sodium orthovanadate, a tyrosine phosphatase inhibitor, increased phosphorylation of tyrosines at positions 1230, 1234, and 1235 of c-Met in A549 cells.
|c-Met (Soluble) ELISA Kit
|c-Met [pY1230/1234/1235] ELISA Kit
|c-Met [pY1230/1234/1235] ELISA Kit||96 tests
- High biological activity
- High purity
- Proven compatibility with Gibco® media
Gibco® recombinant proteins are highly purified bioactive proteins. Gibco® cytokines and receptors have been bioassayed with Gibco® media. Get your recombinant proteins from the media company you use and trust.
We offer high-purity recombinant human HGF for use in cell culture applications. Every lot of HGF is tested for bioactivity by demonstrating the dose-dependent proliferation of monkey 4MBr-5 cells.
- Learn More About Gibco® Cytokines and Growth Factors