Introduction

The chicken embryo chorioallantoic membrane assay has been a model for studying neovascularization since the early 1970’s when it was adapted by Folkmann et al.
TOP

Assay Description

While there have been many variations developed over the years, the basic assay is performed by implanting a membrane or coverslip containing the compound of interest on the chick embryo chorioallantoic membrane through a hole cut in the egg shell. The subsequent incubation period ranges from 1-3 days, depending on the compound, after which time angiogenesis can be quantified via image analysis or colorimetric detection methods. 
  
The CAM assay is quick, technically simple, and inexpensive. However, a major drawback is that it is labor intensive due to the large number of eggs that are required to obtain consistent results. The investigator should note that this is a non-mammalian system which should be taken into consideration when interpreting results.

Materials

TOP

LT175      updated  7-Oct-2011