NIH 3T3 cells showing incorporation of BrdU.

Cells were pulsed with BrdU (B23151) for 30 min before fixation. BrdU incorporated into the DNA of proliferating cells was detected with an anti-BrdU antibody (A21300) and green-fluorescent Alexa Fluor 488 Goat Anti–Mouse IgG1 isotype–specific secondary antibody (A21121); the BrdU staining pattern is co-localized with the nuclear staining of blue-fluorescent DAPI (D1306, D21490). Mitochondria were labeled with anti–COX Complex IV subunit I antibody and red-fluorescent Alexa Fluor® 594 Goat Anti–Mouse IgG2a isotype-specific antibody (A21135). Coverslips were mounted with Prolong Antifade Mounting Medium (P7481).

Cells were pulsed with BrdU (B23151) for 30 min before fixation. BrdU incorporated into the DNA of proliferating cells was detected with an anti-BrdU antibody (A21300) and green-fluorescent Alexa Fluor 488 Goat Anti–Mouse IgG<sub>1</sub> isotype–specific secondary antibody (A21121); the BrdU staining pattern is co-localized with the nuclear staining of blue-fluorescent DAPI (D1306, D21490). Mitochondria were labeled with anti–COX Complex IV subunit I antibody and red-fluorescent Alexa Fluor® 594 Goat Anti–Mouse IgG<sub>2a</sub> isotype-specific antibody (A21135). Coverslips were mounted with Prolong Antifade Mounting Medium (P7481).

Related Products

Sku Name Size Price Qty
A21135 Alexa Fluor® 594 Goat Anti-Mouse IgG2a (γ2a) 250 µL USD 159.00
B23151 BrdU (5-Bromo-2´-Deoxyuridine) 100 mg USD 96.50
D1306 DAPI (4',6-Diamidino-2-Phenylindole, Dihydrochloride) 10 mg USD 89.00
D21490 DAPI, FluoroPure™ grade 10 mg USD 133.00
P7481 ProLong® Antifade Kit 1 kit USD 241.00

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