Immunocytochemistry analysis using Rb [pSpT249/252] ABfinity™ Recombinant Rabbit Monoclonal Antibody.

Immunocytochemistry analysis of U2OS cells stained with Rb [pSpT249/252] ABfinity™ Recombinant Rabbit Monoclonal Antibody, using (A) Alexa Fluor® 488 Goat Anti-Rabbit was used as secondary (green). (B) DAPI was used to stain the nucleus (blue) and (C) Alexa Fluor® 594 phalloidin was used to stain actin (red). (D) Composite image of cells showing nuclear localization of phosphorylated Rb. (E) Composite image of cells showing competition with the phospho Rb [pSpT249/252].

Immunocytochemistry analysis of U2OS cells stained with Rb [pSpT249/252] ABfinity™ Recombinant Rabbit Monoclonal Antibody, using (A) Alexa Fluor® 488 Goat Anti-Rabbit was used as secondary (green). (B) DAPI was used to stain the nucleus (blue) and (C) Alexa Fluor® 594 phalloidin was used to stain actin (red). (D) Composite image of cells showing nuclear localization of phosphorylated Rb. (E) Composite image of cells showing competition with the phospho Rb [pSpT249/252].

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