Endocytosis of EGF in A431 cells
A431 cells were loaded with 5 µg/mL pHrodo™ Red EGF Conjugate (Cat. No. P35374) at 37°C for 30 minutes. Cells on right were pretreated with 25 µg/mL unlabeled EGF, which internalized most of the EGF receptors from the surface, thus reducing the staining of pHrodo™ Red EGF Conjugate. Images were taken by a fluorescent microscope, while using standard TRITC filter set.
Human peripheral blood leukocytes stained with CD45 FITC and antibody clone 581 PerCP-Cy®5.5 conjugate (top, Cat. No. A14949) or mouse IgG1,kappa PerCP-Cy®5.5 isotype control (bottom). Cy®togram gated for total live CD14 negative lymphocyte population. Go ›
Human peripheral blood leukocytes stained with CD34 antibody (clone 581) Pacific Blue conjugate (top, Cat. No. A14947) or mouse IgG1,kappa Pacific Blue isotype control (bottom) and costained with CD45 FITC, CD14 PE, and 7-AAD, then analyzed via gating on total live and CD14-negative lymphocyte population. Go ›