Multi-color, multi-day image analysis using the CellTracker™ Blue, Green, and Red dyes

Using the CellTracker® dyes, three different cell types were labeled. HeLa cells were labeled with CellTracker® Blue CMAC dye (C2110); A549 cells were labeled with CellTracker® Red CMTPX (C34552); and BPAE cells were labeled with CellTracker® Green CMFDA (C2925). After labeling, the three cell types were mixed and co-cultured. After three days of co-culture, images from the DAPI, FITC, and Texas Red® channels were obtained and overlaid. A) 10X image, B) 20X image of the co-culture.

Using the CellTracker® dyes, three different cell types were labeled.  HeLa cells were labeled with CellTracker® Blue CMAC dye (C2110); A549 cells were labeled with CellTracker® Red CMTPX (C34552); and BPAE cells were labeled with CellTracker® Green CMFDA (C2925).  After labeling, the three cell types were mixed and co-cultured.  After three days of co-culture, images from the DAPI, FITC, and Texas Red® channels were obtained and overlaid.  A) 10X image, B) 20X image of the co-culture.

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