Primary Mouse Neurons can be maintained at least for 3 weeks
Life Technologies’ Primary Mouse Cortical Neurons (Cat. No. A15585) were cultured on to poly-D-lysine coated plates for 7 days in Neurobasal® media supplemented with 2% B-27® (Cat. No 17504-044) and 0.5 mM GLUTAMAX™-I (Cat. No. 35050). Immunocytochemistry was performed at Day 14 (left) and Day 24 (right). Cells were stained with mouse anti-MAP2 antibody and Alexa fluor® 488–labeled anti-mouse secondary antibody. Astrocytes were stained with rabbit anti-GFAP antibody and Alexa fluor® 594–labeled rabbit secondary antibody. Nuclei were stained with DAPI.
Human peripheral blood leukocytes stained with CD45 FITC and antibody clone 581 PerCP-Cy®5.5 conjugate (top, Cat. No. A14949) or mouse IgG1,kappa PerCP-Cy®5.5 isotype control (bottom). Cy®togram gated for total live CD14 negative lymphocyte population. Go ›
Human peripheral blood leukocytes stained with CD34 antibody (clone 581) Pacific Blue conjugate (top, Cat. No. A14947) or mouse IgG1,kappa Pacific Blue isotype control (bottom) and costained with CD45 FITC, CD14 PE, and 7-AAD, then analyzed via gating on total live and CD14-negative lymphocyte population. Go ›