Cryopreserved mouse hippocampal neurons were able to be transfected and transduced effectively
Primary Mouse Hippocampal Neurons (Cat. No. A15587) labeled with EGFP control using Lipofectamine® 2000 (Cat. No. 11668) (left), and with BacMam GFP Transduction Control (BacMam 2.0; Cat. No. B10383) (right). Cells were cultured on to poly-D-lysine coated plates for 7 days in Neurobasal® media supplemented with 2% B27 (Cat. No 17504) and 0.5 mM GLUTAMAX™-I (Cat. No. 35050).
Human peripheral blood leukocytes stained with CD45 FITC and antibody clone 581 PerCP-Cy®5.5 conjugate (top, Cat. No. A14949) or mouse IgG1,kappa PerCP-Cy®5.5 isotype control (bottom). Cy®togram gated for total live CD14 negative lymphocyte population. Go ›
Human peripheral blood leukocytes stained with CD34 antibody (clone 581) Pacific Blue conjugate (top, Cat. No. A14947) or mouse IgG1,kappa Pacific Blue isotype control (bottom) and costained with CD45 FITC, CD14 PE, and 7-AAD, then analyzed via gating on total live and CD14-negative lymphocyte population. Go ›